Fodrin and band 4.1 in a plasma membrane-associated fraction of human neutrophils

K. B. Stevenson, Robert A Clark, W. M. Nauseef

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Erythrocytes possess a well-characterized submembranous filamentous network which interacts with transmembrane glycoproteins and is composed primarily of spectrin, ankyrin, band 4.1, and short actin filaments. An analogous structure was recently described in platelets. Human polymorphonuclear leukocytes (PMNs) were examined for the presence and plasma membrane association of similar proteins. Isolated PMNs, free of contamination with erythrocytes or platelets, were disrupted by nitrogen cavitation and separated into subcellular organelles on a discontinuous Percoll gradient. Detergent lysates of plasma membrane vesicles, but not azurophilic or specific granules, contained insoluble actin filaments and associated proteins. Immunoblots of detergent-insoluble plasma membrane fractions contained proteins recognized by antibodies to brain fodrin and erythrocyte band 4.1, whereas blots probed with antibodies to erythrocyte spectrin and ankyrin were negative. Fodrin and band 4.1 were not detected in granule fractions, but some fodrin was present in the cytosol. The assocation of proteins related to fodrin and band 4.1 with the plasma membrane suggests that PMNs contain a submembranous skeleton structurally analogous to that of erythrocytes and platelets. The specific function of these proteins and their structural organization in human PMNs await further study.

Original languageEnglish (US)
Pages (from-to)2136-2143
Number of pages8
JournalBlood
Volume74
Issue number6
StatePublished - 1989
Externally publishedYes

Fingerprint

Cell membranes
Neutrophils
Erythrocytes
Cell Membrane
Platelets
Ankyrins
Spectrin
Blood Platelets
Proteins
Actin Cytoskeleton
Detergents
Actins
Antibodies
Cavitation
Skeleton
Organelles
Cytosol
Brain
Glycoproteins
Contamination

ASJC Scopus subject areas

  • Hematology

Cite this

Fodrin and band 4.1 in a plasma membrane-associated fraction of human neutrophils. / Stevenson, K. B.; Clark, Robert A; Nauseef, W. M.

In: Blood, Vol. 74, No. 6, 1989, p. 2136-2143.

Research output: Contribution to journalArticle

Stevenson, KB, Clark, RA & Nauseef, WM 1989, 'Fodrin and band 4.1 in a plasma membrane-associated fraction of human neutrophils', Blood, vol. 74, no. 6, pp. 2136-2143.
Stevenson, K. B. ; Clark, Robert A ; Nauseef, W. M. / Fodrin and band 4.1 in a plasma membrane-associated fraction of human neutrophils. In: Blood. 1989 ; Vol. 74, No. 6. pp. 2136-2143.
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