@inbook{229904bd0ccd4d629253c77ad28f5764,
title = "Fluorescent Imaging of Cancer in Zebrafish",
abstract = "Zebrafish are an ideal model organism to research cancer. Zebrafish embryos and larvae are optically translucent, which has made imaging multiple processes in development and disease possible. When coupled with fluorescent imaging techniques, zebrafish are fast becoming a model of choice for following tumor formation. This is highlighted by recent studies using fluorescent proteins to image xenograft transplantation, neovascularization, growth responses to drug treatments, and self-renewal. Fluorescent labeled tumors can be generated in zebrafish by multiple methods including chemical mutagenesis, oncogene expression by mosaic or stable transgenesis, or genetic mutations that are predisposing to cancer. In this chapter, we highlight the studies that have employed fluorescence to follow critical aspects of tumorigenesis, with particular focus on providing methods for labeling, isolating, transplanting, and imaging fluorescently labeled tumors in zebrafish.",
keywords = "Confocal microscopy, Irradiated, Labeling, Mutagenesis, Recipient, Regulation",
author = "Ignatius, {Myron S.} and Langenau, {David M.}",
note = "Funding Information: The authors thank Sergei Revskoy for sharing his work and high-resolution images that are reproduced here. David Langenau is supported by NIH grants K01AR05562190-01A1 and K01AR05562190-S1, a new investigator grant from Alex Lemonade Stand, the Sarcoma Foundation of America, the Leukemia Research Foundation, and a seed grant from the Harvard Stem Cell Institute.",
year = "2011",
doi = "10.1016/B978-0-12-381320-6.00019-9",
language = "English (US)",
series = "Methods in Cell Biology",
publisher = "Academic Press Inc.",
pages = "437--459",
booktitle = "Methods in Cell Biology",
address = "United States",
}