Fluorescent Imaging of Cancer in Zebrafish

Myron S. Ignatius, David M. Langenau

Research output: Chapter in Book/Report/Conference proceedingChapter

14 Scopus citations

Abstract

Zebrafish are an ideal model organism to research cancer. Zebrafish embryos and larvae are optically translucent, which has made imaging multiple processes in development and disease possible. When coupled with fluorescent imaging techniques, zebrafish are fast becoming a model of choice for following tumor formation. This is highlighted by recent studies using fluorescent proteins to image xenograft transplantation, neovascularization, growth responses to drug treatments, and self-renewal. Fluorescent labeled tumors can be generated in zebrafish by multiple methods including chemical mutagenesis, oncogene expression by mosaic or stable transgenesis, or genetic mutations that are predisposing to cancer. In this chapter, we highlight the studies that have employed fluorescence to follow critical aspects of tumorigenesis, with particular focus on providing methods for labeling, isolating, transplanting, and imaging fluorescently labeled tumors in zebrafish.

Original languageEnglish (US)
Title of host publicationMethods in Cell Biology
PublisherAcademic Press Inc.
Pages437-459
Number of pages23
DOIs
StatePublished - 2011
Externally publishedYes

Publication series

NameMethods in Cell Biology
Volume105
ISSN (Print)0091-679X

Keywords

  • Confocal microscopy
  • Irradiated
  • Labeling
  • Mutagenesis
  • Recipient
  • Regulation

ASJC Scopus subject areas

  • Cell Biology

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  • Cite this

    Ignatius, M. S., & Langenau, D. M. (2011). Fluorescent Imaging of Cancer in Zebrafish. In Methods in Cell Biology (pp. 437-459). (Methods in Cell Biology; Vol. 105). Academic Press Inc.. https://doi.org/10.1016/B978-0-12-381320-6.00019-9