Fluorescent Imaging of Cancer in Zebrafish

Myron S Ignatius, David M. Langenau

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Zebrafish are an ideal model organism to research cancer. Zebrafish embryos and larvae are optically translucent, which has made imaging multiple processes in development and disease possible. When coupled with fluorescent imaging techniques, zebrafish are fast becoming a model of choice for following tumor formation. This is highlighted by recent studies using fluorescent proteins to image xenograft transplantation, neovascularization, growth responses to drug treatments, and self-renewal. Fluorescent labeled tumors can be generated in zebrafish by multiple methods including chemical mutagenesis, oncogene expression by mosaic or stable transgenesis, or genetic mutations that are predisposing to cancer. In this chapter, we highlight the studies that have employed fluorescence to follow critical aspects of tumorigenesis, with particular focus on providing methods for labeling, isolating, transplanting, and imaging fluorescently labeled tumors in zebrafish.

Original languageEnglish (US)
Pages (from-to)437-459
Number of pages23
JournalMethods in Cell Biology
Volume105
DOIs
StatePublished - Sep 30 2011
Externally publishedYes

Fingerprint

Zebrafish
Neoplasms
Gene Transfer Techniques
Heterologous Transplantation
Oncogenes
Mutagenesis
Larva
Carcinogenesis
Embryonic Structures
Fluorescence
Mutation
Growth
Research
Pharmaceutical Preparations
Proteins

Keywords

  • Confocal microscopy
  • Irradiated
  • Labeling
  • Mutagenesis
  • Recipient
  • Regulation

ASJC Scopus subject areas

  • Cell Biology

Cite this

Fluorescent Imaging of Cancer in Zebrafish. / Ignatius, Myron S; Langenau, David M.

In: Methods in Cell Biology, Vol. 105, 30.09.2011, p. 437-459.

Research output: Contribution to journalArticle

Ignatius, Myron S ; Langenau, David M. / Fluorescent Imaging of Cancer in Zebrafish. In: Methods in Cell Biology. 2011 ; Vol. 105. pp. 437-459.
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