Fluorescence-based approaches for quantitative assessment of protein carbonylation, protein disulfides, and protein conformation in biological tissues

Asish R. Chaudhuri, Rochelle Wei, Arunabh Bhattacharya, Ryan Hamilton

Research output: Chapter in Book/Report/Conference proceedingChapter

1 Scopus citations

Abstract

Protein oxidation and misfolding have been considered as key players for progression of aging and etiology of various pathological conditions. However, few attempts have been made to develop sensitive and reproducible assays to quantify the changes in protein oxidation and alteration in structure. Here we describe three distinct fluorescence-based assays to quantify changes in protein oxidation, namely carbonylation and disulfides and alteration in protein surface hydrophobicity as a reporter for protein conformation. These techniques will provide investigators the opportunity to address important biological questions in their experimental models.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press
Pages155-173
Number of pages19
Volume1343
DOIs
StatePublished - 2015
Externally publishedYes

Publication series

NameMethods in Molecular Biology
Volume1343
ISSN (Print)10643745

Keywords

  • Carbonylation
  • Disulfide
  • Oxidative stress
  • Protein oxidation

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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