Fluorescence-based approaches for quantitative assessment of protein carbonylation, protein disulfides, and protein conformation in biological tissues

Asish R. Chaudhuri, Rochelle Wei, Arunabh Bhattacharya, Ryan Hamilton

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Protein oxidation and misfolding have been considered as key players for progression of aging and etiology of various pathological conditions. However, few attempts have been made to develop sensitive and reproducible assays to quantify the changes in protein oxidation and alteration in structure. Here we describe three distinct fluorescence-based assays to quantify changes in protein oxidation, namely carbonylation and disulfides and alteration in protein surface hydrophobicity as a reporter for protein conformation. These techniques will provide investigators the opportunity to address important biological questions in their experimental models.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press
Pages155-173
Number of pages19
Volume1343
DOIs
StatePublished - 2015
Externally publishedYes

Publication series

NameMethods in Molecular Biology
Volume1343
ISSN (Print)10643745

Fingerprint

Protein Carbonylation
Protein Conformation
Disulfides
Fluorescence
Proteins
Hydrophobic and Hydrophilic Interactions
Membrane Proteins
Theoretical Models
Research Personnel

Keywords

  • Carbonylation
  • Disulfide
  • Oxidative stress
  • Protein oxidation

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

Chaudhuri, A. R., Wei, R., Bhattacharya, A., & Hamilton, R. (2015). Fluorescence-based approaches for quantitative assessment of protein carbonylation, protein disulfides, and protein conformation in biological tissues. In Methods in Molecular Biology (Vol. 1343, pp. 155-173). (Methods in Molecular Biology; Vol. 1343). Humana Press. https://doi.org/10.1007/978-1-4939-2963-4_13

Fluorescence-based approaches for quantitative assessment of protein carbonylation, protein disulfides, and protein conformation in biological tissues. / Chaudhuri, Asish R.; Wei, Rochelle; Bhattacharya, Arunabh; Hamilton, Ryan.

Methods in Molecular Biology. Vol. 1343 Humana Press, 2015. p. 155-173 (Methods in Molecular Biology; Vol. 1343).

Research output: Chapter in Book/Report/Conference proceedingChapter

Chaudhuri, AR, Wei, R, Bhattacharya, A & Hamilton, R 2015, Fluorescence-based approaches for quantitative assessment of protein carbonylation, protein disulfides, and protein conformation in biological tissues. in Methods in Molecular Biology. vol. 1343, Methods in Molecular Biology, vol. 1343, Humana Press, pp. 155-173. https://doi.org/10.1007/978-1-4939-2963-4_13
Chaudhuri AR, Wei R, Bhattacharya A, Hamilton R. Fluorescence-based approaches for quantitative assessment of protein carbonylation, protein disulfides, and protein conformation in biological tissues. In Methods in Molecular Biology. Vol. 1343. Humana Press. 2015. p. 155-173. (Methods in Molecular Biology). https://doi.org/10.1007/978-1-4939-2963-4_13
Chaudhuri, Asish R. ; Wei, Rochelle ; Bhattacharya, Arunabh ; Hamilton, Ryan. / Fluorescence-based approaches for quantitative assessment of protein carbonylation, protein disulfides, and protein conformation in biological tissues. Methods in Molecular Biology. Vol. 1343 Humana Press, 2015. pp. 155-173 (Methods in Molecular Biology).
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