TY - JOUR
T1 - Fluconazole disk diffusion susceptibility testing of Candida species
AU - Kirkpatrick, William R.
AU - Turner, Thomas M.
AU - Fothergill, Annette W.
AU - McCarthy, Dora I.
AU - Redding, Spencer W.
AU - Rinaldi, Michael G.
AU - Patterson, Thomas F.
PY - 1998/11
Y1 - 1998/11
N2 - We describe a simple procedure for detecting fluconazole-resistant yeasts by a disk diffusion method. Forty clinical Candida sp. isolates were tested on RPMI-glucose agar with either 25- or 50-μg fluconazole disks. With 25-μg disks, zones of inhibition of ≥20 mm at 24 h accurately identified 29 of 29 isolates for which MICs were ≤8 μg/ml, and with 50-μg disks, zones of ≥27 mm identified 28 of 29 such isolates. All 11 isolates for which MICs were >8 μg/ml were identified by using either disk. Disk diffusion may be a useful screening method for clinical microbiology laboratories.
AB - We describe a simple procedure for detecting fluconazole-resistant yeasts by a disk diffusion method. Forty clinical Candida sp. isolates were tested on RPMI-glucose agar with either 25- or 50-μg fluconazole disks. With 25-μg disks, zones of inhibition of ≥20 mm at 24 h accurately identified 29 of 29 isolates for which MICs were ≤8 μg/ml, and with 50-μg disks, zones of ≥27 mm identified 28 of 29 such isolates. All 11 isolates for which MICs were >8 μg/ml were identified by using either disk. Disk diffusion may be a useful screening method for clinical microbiology laboratories.
UR - https://www.scopus.com/pages/publications/0031717756
UR - https://www.scopus.com/pages/publications/0031717756#tab=citedBy
U2 - 10.1128/jcm.36.11.3429-3432.1998
DO - 10.1128/jcm.36.11.3429-3432.1998
M3 - Article
C2 - 9774615
AN - SCOPUS:0031717756
SN - 0095-1137
VL - 36
SP - 3429
EP - 3432
JO - Journal of clinical microbiology
JF - Journal of clinical microbiology
IS - 11
ER -