Fluconazole disk diffusion susceptibility testing of Candida species

William R. Kirkpatrick, Thomas M. Turner, Annette W. Fothergill, Dora I. McCarthy, Spencer W. Redding, Michael G. Rinaldi, Thomas F. Patterson

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Abstract

We describe a simple procedure for detecting fluconazole-resistant yeasts by a disk diffusion method. Forty clinical Candida sp. isolates were tested on RPMI-glucose agar with either 25- or 50-μg fluconazole disks. With 25-μg disks, zones of inhibition of ≥20 mm at 24 h accurately identified 29 of 29 isolates for which MICs were ≤8 μg/ml, and with 50-μg disks, zones of ≥27 mm identified 28 of 29 such isolates. All 11 isolates for which MICs were >8 μg/ml were identified by using either disk. Disk diffusion may be a useful screening method for clinical microbiology laboratories.

Original languageEnglish (US)
Pages (from-to)3429-3432
Number of pages4
JournalJournal of clinical microbiology
Volume36
Issue number11
StatePublished - Nov 1 1998

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ASJC Scopus subject areas

  • Microbiology (medical)

Cite this

Kirkpatrick, W. R., Turner, T. M., Fothergill, A. W., McCarthy, D. I., Redding, S. W., Rinaldi, M. G., & Patterson, T. F. (1998). Fluconazole disk diffusion susceptibility testing of Candida species. Journal of clinical microbiology, 36(11), 3429-3432.