Fluconazole disk diffusion susceptibility testing of Candida species

William R. Kirkpatrick, Thomas M. Turner, Annette W. Fothergill, Dora I. McCarthy, Spencer W. Redding, Michael G. Rinaldi, Thomas F. Patterson

Research output: Contribution to journalArticlepeer-review

73 Scopus citations


We describe a simple procedure for detecting fluconazole-resistant yeasts by a disk diffusion method. Forty clinical Candida sp. isolates were tested on RPMI-glucose agar with either 25- or 50-μg fluconazole disks. With 25-μg disks, zones of inhibition of ≥20 mm at 24 h accurately identified 29 of 29 isolates for which MICs were ≤8 μg/ml, and with 50-μg disks, zones of ≥27 mm identified 28 of 29 such isolates. All 11 isolates for which MICs were >8 μg/ml were identified by using either disk. Disk diffusion may be a useful screening method for clinical microbiology laboratories.

Original languageEnglish (US)
Pages (from-to)3429-3432
Number of pages4
JournalJournal of clinical microbiology
Issue number11
StatePublished - Nov 1998

ASJC Scopus subject areas

  • Microbiology (medical)


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