Flow Cytometric Measurement of Rat Lymphocyte Subpopulations After Burn Injury and Burn Injury With Infection

David G. Burleson, George K. Vaughn, Arthur D. Mason, Basil A. Pruitt

Research output: Contribution to journalArticlepeer-review

18 Scopus citations

Abstract

• Increased infection rates in burned patients may result from a disproportionate increase in the suppressor subpopulations. Measurement of lymphocyte subpopulations is difficult in burned patients because gradient-purified cells are contaminated by nonlymphoid cells. The accuracy of flow cytometric subpopulation analysis was improved by restricting (gating) the analysis to cells with light-scatter intensity typical of lymphocytes. Blood was obtained 48 hours after burn from rats receiving no burns, 30% scald burns, or burns seeded with Pseudomonas aeruginosa to induce infection. Subpopulations were identified by monoclonal antibodies to T-lymphocyte antigens. Gating increased the values obtained for most subpopulations, but the relative differences between groups were unchanged. Burned and infected animals, but not animals burned only, had a decreased ratio of helper to suppressor lymphocytes (HSR) relative to control. A decreased HSR correlated with sepsis, but not with infection susceptibility. This suggests that a decrease in HSR may be a result of infection rather than a cause of susceptibility to infection.

Original languageEnglish (US)
Pages (from-to)216-220
Number of pages5
JournalArchives of Surgery
Volume122
Issue number2
DOIs
StatePublished - Feb 1987

ASJC Scopus subject areas

  • Surgery

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