Filoviruses utilize glycosaminoglycans for their attachment to target cells

Beatriz Salvador, Nicole R. Sexton, Ricardo Carrion, Jerritt Nunneley, Jean L. Patterson, Imke Steffen, Kai Lu, Marcus O. Muench, David Lembo, Graham Simmons

Research output: Contribution to journalArticle

44 Citations (Scopus)

Abstract

Filoviruses are the cause of severe hemorrhagic fever in human and nonhuman primates. The envelope glycoprotein (GP), responsible for both receptor binding and fusion of the virus envelope with the host cell membrane, has been demonstrated to interact with multiple molecules in order to enhance entry into host cells. Here we have demonstrated that filoviruses utilize glycosaminoglycans, and more specifically heparan sulfate proteoglycans, for their attachment to host cells. This interaction is mediated by GP and does not require the presence of the mucin domain. Both the degree of sulfation and the structure of the carbohydrate backbone play a role in the interaction with filovirus GPs. This new step of filovirus interaction with host cells can potentially be a new target for antiviral strategies. As such, we were able to inhibit filovirus GP-mediated infection using carrageenan, a broad-spectrum microbicide that mimics heparin, and also using the antiviral dendrimeric peptide SB105-A10, which interacts with heparan sulfate, antagonizing the binding of the virus to cells.

Original languageEnglish (US)
Pages (from-to)3295-3304
Number of pages10
JournalJournal of Virology
Volume87
Issue number6
DOIs
StatePublished - Mar 2013
Externally publishedYes

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Filoviridae
glycosaminoglycans
Glycosaminoglycans
Virus Attachment
Glycoproteins
glycoproteins
Antiviral Agents
cells
Heparan Sulfate Proteoglycans
Heparitin Sulfate
carbohydrate structure
Carrageenan
Mucins
Anti-Infective Agents
viruses
proteoglycans
Primates
mucins
carrageenan
Heparin

ASJC Scopus subject areas

  • Immunology
  • Virology

Cite this

Salvador, B., Sexton, N. R., Carrion, R., Nunneley, J., Patterson, J. L., Steffen, I., ... Simmons, G. (2013). Filoviruses utilize glycosaminoglycans for their attachment to target cells. Journal of Virology, 87(6), 3295-3304. https://doi.org/10.1128/JVI.01621-12

Filoviruses utilize glycosaminoglycans for their attachment to target cells. / Salvador, Beatriz; Sexton, Nicole R.; Carrion, Ricardo; Nunneley, Jerritt; Patterson, Jean L.; Steffen, Imke; Lu, Kai; Muench, Marcus O.; Lembo, David; Simmons, Graham.

In: Journal of Virology, Vol. 87, No. 6, 03.2013, p. 3295-3304.

Research output: Contribution to journalArticle

Salvador, B, Sexton, NR, Carrion, R, Nunneley, J, Patterson, JL, Steffen, I, Lu, K, Muench, MO, Lembo, D & Simmons, G 2013, 'Filoviruses utilize glycosaminoglycans for their attachment to target cells', Journal of Virology, vol. 87, no. 6, pp. 3295-3304. https://doi.org/10.1128/JVI.01621-12
Salvador B, Sexton NR, Carrion R, Nunneley J, Patterson JL, Steffen I et al. Filoviruses utilize glycosaminoglycans for their attachment to target cells. Journal of Virology. 2013 Mar;87(6):3295-3304. https://doi.org/10.1128/JVI.01621-12
Salvador, Beatriz ; Sexton, Nicole R. ; Carrion, Ricardo ; Nunneley, Jerritt ; Patterson, Jean L. ; Steffen, Imke ; Lu, Kai ; Muench, Marcus O. ; Lembo, David ; Simmons, Graham. / Filoviruses utilize glycosaminoglycans for their attachment to target cells. In: Journal of Virology. 2013 ; Vol. 87, No. 6. pp. 3295-3304.
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