TY - JOUR
T1 - Fibronectin-binding protein TDE1579 affects cytotoxicity of Treponema denticola
AU - Xu, Xiaoping
AU - Steffensen, Bjorn
AU - Robichaud, Trista K.
AU - Mikhailova, Margarita
AU - Lai, Veronica
AU - Montgomery, Ryan
AU - Chu, Lianrui
N1 - Funding Information:
We gratefully acknowledge the Nucleic Acids Core Facility at the UTHSCSA for primers syntheses and DNA sequencing. We also thank Drs. Zhihua Chen and Sanjay Pal in Department of Periodontics, UTHSCSA for the expression and purification of recombinant fibronectin fragments, Dr. Susan Weintraub and Kevin Hakala at the UTHSCSA Mass Spectrometry Laboratory for mass spectrometry analysis and Barbara Hunter at Department of Pathology, UTHSCSA for electronmicroscopy observation. This work was supported by NIDCR grants DE021520 , DE018135 , DE017139 , and DE014318 for COSTAR.
PY - 2015/12/1
Y1 - 2015/12/1
N2 - While FbpA, a family of bacterial fibronectin (FN) binding proteins has been studied in several gram-positive bacteria, the gram-negative Treponema denticola, an anaerobic periodontal pathogen, also has an overlooked fbp gene (tde1579). In this research, we confirm that recombinant Fbp protein (rFbp) of T. denticola binds human FN with a Kdapp of 1.5 × 10-7 M and blocks the binding of T. denticola to FN in a concentration-dependent manner to a level of 42%. The fbp gene was expressed in T. denticola. To reveal the roles of fbp in T. denticola pathogenesis, an fbp isogenic mutant was constructed. The fbp mutant had 51% reduced binding ability to human gingival fibroblasts (hGF). When hGF were challenged with T. denticola, the fbp mutant caused less cell morphology change, had 50% reduced cytotoxicity to hGF, and had less influence on the growth of hGF cells.
AB - While FbpA, a family of bacterial fibronectin (FN) binding proteins has been studied in several gram-positive bacteria, the gram-negative Treponema denticola, an anaerobic periodontal pathogen, also has an overlooked fbp gene (tde1579). In this research, we confirm that recombinant Fbp protein (rFbp) of T. denticola binds human FN with a Kdapp of 1.5 × 10-7 M and blocks the binding of T. denticola to FN in a concentration-dependent manner to a level of 42%. The fbp gene was expressed in T. denticola. To reveal the roles of fbp in T. denticola pathogenesis, an fbp isogenic mutant was constructed. The fbp mutant had 51% reduced binding ability to human gingival fibroblasts (hGF). When hGF were challenged with T. denticola, the fbp mutant caused less cell morphology change, had 50% reduced cytotoxicity to hGF, and had less influence on the growth of hGF cells.
KW - Fbp
KW - Fibronectin binding protein
KW - Gene mutant
KW - Periodontal disease
KW - Treponema denticola
UR - http://www.scopus.com/inward/record.url?scp=84944185423&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84944185423&partnerID=8YFLogxK
U2 - 10.1016/j.anaerobe.2015.09.010
DO - 10.1016/j.anaerobe.2015.09.010
M3 - Article
C2 - 26456217
AN - SCOPUS:84944185423
VL - 36
SP - 39
EP - 48
JO - Anaerobe
JF - Anaerobe
SN - 1075-9964
ER -