Fibronectin-binding protein TDE1579 affects cytotoxicity of Treponema denticola

Xiaoping Xu, Bjorn Steffensen, Trista K. Robichaud, Margarita Mikhailova, Veronica Lai, Ryan Montgomery, Lianrui Chu

Research output: Contribution to journalArticlepeer-review

1 Scopus citations


While FbpA, a family of bacterial fibronectin (FN) binding proteins has been studied in several gram-positive bacteria, the gram-negative Treponema denticola, an anaerobic periodontal pathogen, also has an overlooked fbp gene (tde1579). In this research, we confirm that recombinant Fbp protein (rFbp) of T. denticola binds human FN with a Kdapp of 1.5 × 10-7 M and blocks the binding of T. denticola to FN in a concentration-dependent manner to a level of 42%. The fbp gene was expressed in T. denticola. To reveal the roles of fbp in T. denticola pathogenesis, an fbp isogenic mutant was constructed. The fbp mutant had 51% reduced binding ability to human gingival fibroblasts (hGF). When hGF were challenged with T. denticola, the fbp mutant caused less cell morphology change, had 50% reduced cytotoxicity to hGF, and had less influence on the growth of hGF cells.

Original languageEnglish (US)
Pages (from-to)39-48
Number of pages10
StatePublished - Dec 1 2015


  • Fbp
  • Fibronectin binding protein
  • Gene mutant
  • Periodontal disease
  • Treponema denticola

ASJC Scopus subject areas

  • Microbiology
  • Infectious Diseases


Dive into the research topics of 'Fibronectin-binding protein TDE1579 affects cytotoxicity of Treponema denticola'. Together they form a unique fingerprint.

Cite this