Fibroblasts from naked mole-rats are resistant to multiple forms of cell injury, but sensitive to peroxide, ultraviolet light, and endoplasmic reticulum stress

Adam B. Salmon, Amir A. Sadighi Akha, Rochelle Buffenstein, Richard A. Miller

Research output: Contribution to journalArticlepeer-review

86 Scopus citations

Abstract

Fibroblasts from long-lived mutant mice are resistant to many forms of lethal injury as well as to the metabolic effects of rotenone and low-glucose medium. Here we evaluated fibroblasts from young adult naked mole-rats (NMR; Heterocephalus glaber), a rodent species in which maximal longevity exceeds 28 years. Compared to mouse cells, NMR cells were resistant to cadmium, methyl methanesulfonate, paraquat, heat, and low-glucose medium, consistent with the idea that cellular resistance to stress may contribute to disease resistance and longevity. Surprisingly, NMR cells were more sensitive than mouse cells to H2O2, ultraviolet (UV) light, and rotenone. NMR cells, like cells from Snell dwarf mice, were more sensitive to tunicamycin and thapsigargin, which interfere with the function of the endoplasmic reticulum (ER stress). The sensitivity of both Snell dwarf and NMR cells to ER stress suggests that alterations in the unfolded protein response might modulate cell survival and aging rate.

Original languageEnglish (US)
Pages (from-to)232-241
Number of pages10
JournalJournals of Gerontology - Series A Biological Sciences and Medical Sciences
Volume63
Issue number3
DOIs
StatePublished - Mar 2008

Keywords

  • Comparative biology
  • Endoplasmic reticulum (ER) stress
  • Longevity
  • Naked mole-rat
  • Oxidation
  • Stress resistance

ASJC Scopus subject areas

  • Aging
  • Geriatrics and Gerontology

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