Fibrinolytic activity in bronchoalveolar lavage of baboons with diffuse alveolar damage: Trends in two forms of lung injury

S. Idell, K. K. James, J. J. Coalson

Research output: Contribution to journalArticle

27 Scopus citations

Abstract

Background and Methods: Alveolar fibrin deposition is prominent in diffuse alveolar damage, the morphologic hallmark of the adult respiratory distress syndrome. To determine if a persistent abnormality of fibrin clearance occurs in the alveolar compartment during evolving diffuse alveolar damage, we characterized abnormalities of fibrin turnover in serial bronchoalveolar lavage specimens from two baboon models: a) diffuse alveolar damage induced by 80% oxygen and bronchoscopic seeding of Pseudomonas aeruginosa; and b) a more fulminant form of diffuse alveolar damage induced by bronchoscopic seeding of Pseudomonas and the infusion of oleic acid. Results: Lavage procoagulant activity, due mainly to tissue factor associated with Factor VII, was increased and exceeded regulation by extrinsic pathway inhibitor in both models. Fibrinolytic activity was transiently diminished in baboons with evolving diffuse alveolar damage induced by oleic acid/Pseudomonas, but was preserved after 80% oxygen/Pseudomonas. Concentrations of plasminogen activator inhibitor-2 did not increase in lavage specimens obtained during evolving diffuse alveolar damage. Concentrations of α2 antiplasmin and plasminogen activator inhibitor-1 tended to be higher in the lavage of oleic acid/Pseudomonas baboons with low fibrinolytic activity. Immunohistochemical analyses showed that tissue factor was distributed along the alveolar surface of controls and baboons with diffuse alveolar damage. Alveolar fibrin deposition was increased, by morphometric analyses, in both models. Conclusions: These data indicate that while increased procoagulant activity is characteristic of evolving diffuse alveolar damage and favors alveolar fibrin deposition, fibrinolytic activity may be transiently diminished or remain intact during evolving diffuse alveolar damage in baboons.

Original languageEnglish (US)
Pages (from-to)1431-1440
Number of pages10
JournalCritical care medicine
Volume20
Issue number10
DOIs
StatePublished - 1992

Keywords

  • Factor VII
  • antiplasmin
  • bronchoalveolar lavage fluid
  • fibrinolysis
  • plasminogen activator, tissue-type
  • plasminogen inactivators
  • respiratory distress syndrome, adult
  • thromboplastin
  • urokinase

ASJC Scopus subject areas

  • Critical Care and Intensive Care Medicine

Fingerprint Dive into the research topics of 'Fibrinolytic activity in bronchoalveolar lavage of baboons with diffuse alveolar damage: Trends in two forms of lung injury'. Together they form a unique fingerprint.

  • Cite this