Fibrin hydrogels to deliver dental stem cells of the apical papilla for regenerative medicine

Loïc Germain; Pauline De Berdt; Julie Vanacker; Julian Leprince; Anibal Diogenes; Damien Jacobs; Gaëlle Vandermeulen; Caroline Bouzin; Véronique Préat; Christine Dupont-Gillain; Anne Des Rieux

doi:10.2217/rme.14.81

Fibrin hydrogels to deliver dental stem cells of the apical papilla for regenerative medicine

Loïc Germain, Pauline De Berdt, Julie Vanacker, Julian Leprince, Anibal Diogenes, Damien Jacobs, Gaëlle Vandermeulen, Caroline Bouzin, Véronique Préat, Christine Dupont-Gillain, Anne Des Rieux

Department of Endodontics

Research output: Contribution to journal › Article › peer-review

22 Scopus citations

Abstract

Aim: Evaluation of survival, proliferation and neurodifferentiation of dental stem cells from the apical papilla (SCAP) in fibrin hydrogels. We hypothesized that fibrin composition will influence cell behavior. Methods: Modulus, pore and fiber size were measured. SCAP in vitro viability, proliferation and neural differentiation, as well as in vivo proliferation and angiogenesis were studied. Results: Hydrogel moduli were influenced by fibrin formulation but not hydrogel morphology, SCAP in vitro viability and proliferation. In total 60% of SCAP expressed PanNeurofilament in vitro without induction in Fibrinogen50-Thrombin10. SCAP proliferated when implanted in vivo and stimulated host endothelial cell infiltration. Conclusion: Fibrinogen30-Thrombin10 or Thrombin50 would be more favorable to in vitro SCAP viability and in vivo proliferation, while Fibrinogen 50-Thrombin50 would be more adapted to neurodifferentiation.

Original language	English (US)
Pages (from-to)	153-167
Number of pages	15
Journal	Regenerative Medicine
Volume	10
Issue number	2
DOIs	https://doi.org/10.2217/rme.14.81
State	Published - Mar 1 2015

Keywords

angiogenesis
dental stem cells
fibrin
neurodifferentiation
tissue engineering

ASJC Scopus subject areas

Biomedical Engineering
Embryology

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10.2217/rme.14.81

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title = "Fibrin hydrogels to deliver dental stem cells of the apical papilla for regenerative medicine",

abstract = "Aim: Evaluation of survival, proliferation and neurodifferentiation of dental stem cells from the apical papilla (SCAP) in fibrin hydrogels. We hypothesized that fibrin composition will influence cell behavior. Methods: Modulus, pore and fiber size were measured. SCAP in vitro viability, proliferation and neural differentiation, as well as in vivo proliferation and angiogenesis were studied. Results: Hydrogel moduli were influenced by fibrin formulation but not hydrogel morphology, SCAP in vitro viability and proliferation. In total 60% of SCAP expressed PanNeurofilament in vitro without induction in Fibrinogen50-Thrombin10. SCAP proliferated when implanted in vivo and stimulated host endothelial cell infiltration. Conclusion: Fibrinogen30-Thrombin10 or Thrombin50 would be more favorable to in vitro SCAP viability and in vivo proliferation, while Fibrinogen 50-Thrombin50 would be more adapted to neurodifferentiation.",

keywords = "angiogenesis, dental stem cells, fibrin, neurodifferentiation, tissue engineering",

author = "Lo{\"i}c Germain and {De Berdt}, Pauline and Julie Vanacker and Julian Leprince and Anibal Diogenes and Damien Jacobs and Ga{\"e}lle Vandermeulen and Caroline Bouzin and V{\'e}ronique Pr{\'e}at and Christine Dupont-Gillain and {Des Rieux}, Anne",

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year = "2015",

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doi = "10.2217/rme.14.81",

language = "English (US)",

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T1 - Fibrin hydrogels to deliver dental stem cells of the apical papilla for regenerative medicine

AU - Germain, Loïc

AU - De Berdt, Pauline

AU - Vanacker, Julie

AU - Leprince, Julian

AU - Diogenes, Anibal

AU - Jacobs, Damien

AU - Vandermeulen, Gaëlle

AU - Bouzin, Caroline

AU - Préat, Véronique

AU - Dupont-Gillain, Christine

AU - Des Rieux, Anne

PY - 2015/3/1

Y1 - 2015/3/1

N2 - Aim: Evaluation of survival, proliferation and neurodifferentiation of dental stem cells from the apical papilla (SCAP) in fibrin hydrogels. We hypothesized that fibrin composition will influence cell behavior. Methods: Modulus, pore and fiber size were measured. SCAP in vitro viability, proliferation and neural differentiation, as well as in vivo proliferation and angiogenesis were studied. Results: Hydrogel moduli were influenced by fibrin formulation but not hydrogel morphology, SCAP in vitro viability and proliferation. In total 60% of SCAP expressed PanNeurofilament in vitro without induction in Fibrinogen50-Thrombin10. SCAP proliferated when implanted in vivo and stimulated host endothelial cell infiltration. Conclusion: Fibrinogen30-Thrombin10 or Thrombin50 would be more favorable to in vitro SCAP viability and in vivo proliferation, while Fibrinogen 50-Thrombin50 would be more adapted to neurodifferentiation.

AB - Aim: Evaluation of survival, proliferation and neurodifferentiation of dental stem cells from the apical papilla (SCAP) in fibrin hydrogels. We hypothesized that fibrin composition will influence cell behavior. Methods: Modulus, pore and fiber size were measured. SCAP in vitro viability, proliferation and neural differentiation, as well as in vivo proliferation and angiogenesis were studied. Results: Hydrogel moduli were influenced by fibrin formulation but not hydrogel morphology, SCAP in vitro viability and proliferation. In total 60% of SCAP expressed PanNeurofilament in vitro without induction in Fibrinogen50-Thrombin10. SCAP proliferated when implanted in vivo and stimulated host endothelial cell infiltration. Conclusion: Fibrinogen30-Thrombin10 or Thrombin50 would be more favorable to in vitro SCAP viability and in vivo proliferation, while Fibrinogen 50-Thrombin50 would be more adapted to neurodifferentiation.

KW - angiogenesis

KW - dental stem cells

KW - fibrin

KW - neurodifferentiation

KW - tissue engineering

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Fibrin hydrogels to deliver dental stem cells of the apical papilla for regenerative medicine

Abstract

Keywords

ASJC Scopus subject areas

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