Abstract
Aim: Evaluation of survival, proliferation and neurodifferentiation of dental stem cells from the apical papilla (SCAP) in fibrin hydrogels. We hypothesized that fibrin composition will influence cell behavior. Methods: Modulus, pore and fiber size were measured. SCAP in vitro viability, proliferation and neural differentiation, as well as in vivo proliferation and angiogenesis were studied. Results: Hydrogel moduli were influenced by fibrin formulation but not hydrogel morphology, SCAP in vitro viability and proliferation. In total 60% of SCAP expressed PanNeurofilament in vitro without induction in Fibrinogen50-Thrombin10. SCAP proliferated when implanted in vivo and stimulated host endothelial cell infiltration. Conclusion: Fibrinogen30-Thrombin10 or Thrombin50 would be more favorable to in vitro SCAP viability and in vivo proliferation, while Fibrinogen 50-Thrombin50 would be more adapted to neurodifferentiation.
Original language | English (US) |
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Pages (from-to) | 153-167 |
Number of pages | 15 |
Journal | Regenerative Medicine |
Volume | 10 |
Issue number | 2 |
DOIs | |
State | Published - Mar 1 2015 |
Keywords
- angiogenesis
- dental stem cells
- fibrin
- neurodifferentiation
- tissue engineering
ASJC Scopus subject areas
- Biomedical Engineering
- Embryology