Fiber optic fluorescence microscopy for functional brain imaging in awake, mobile mice

Jaepyeong Cha, Martin Paukert, Dwight E. Bergles, Jin U. Kang

Research output: Chapter in Book/Report/Conference proceedingConference contribution

4 Scopus citations

Abstract

Fiber-optic based optical imaging is an emerging technique for studying brain activity in live animals. Here, we introduce a novel fluorescence fiber-optic microendoscopy approach to minimal invasively detect neural activities in a live mouse brain. The system uses a flexible endoscopic probe composed of a multi-core coherent fiber-bundle terminated with an approximately 1500-micron working distance objective lens. The fiber-optic neural interface is mounted on a 4-mm2 cranial window enabling visualization of glial calcium transients from the same brain region for weeks. We evaluated the system performance through in vivo imaging of GCaMP3 fluorescence in transgenic headrestrained mice during locomotion.

Original languageEnglish (US)
Title of host publicationOptical Techniques in Neurosurgery, Neurophotonics, and Optogenetics
PublisherSPIE
ISBN (Print)9780819498410
DOIs
StatePublished - Jan 1 2014
EventOptical Techniques in Neurosurgery, Neurophotonics, and Optogenetics - San Francisco, CA, United States
Duration: Feb 1 2014Feb 4 2014

Publication series

NameProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume8928
ISSN (Print)1605-7422

Other

OtherOptical Techniques in Neurosurgery, Neurophotonics, and Optogenetics
Country/TerritoryUnited States
CitySan Francisco, CA
Period2/1/142/4/14

Keywords

  • Astrocyte calcium transients
  • Fiber-optic sensor
  • Fluorescence microendoscopy
  • Functional brain imaging
  • GCaMP3
  • cerebellum

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Atomic and Molecular Physics, and Optics
  • Radiology Nuclear Medicine and imaging

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