Fecal microbiome, metabolites, and stem cell transplant outcomes: A single-center pilot study

Jessica R. Galloway-Peña, Christine B. Peterson, Farida Malik, Pranoti V. Sahasrabhojane, Dimpy P Shah, Chelcy E. Brumlow, Lily G. Carlin, Roy F. Chemaly, Jin Seon Im, Gabriela Rondon, Edd Felix, Lucas Veillon, Philip L. Lorenzi, Amin M. Alousi, Robert R. Jenq, Dimitrios P. Kontoyiannis, Elizabeth J. Shpall, Samuel A. Shelburne, Pablo C. Okhuysen

Research output: Contribution to journalArticle

Abstract

Background. Accumulating evidence suggests that the intestinal microbiome may dramatically affect the outcomes of hematopoietic stem cell transplant (HSCT) recipients. Providing 16S ribosomal RNA based microbiome characterization in a clinically actionable time frame is currently problematic. Thus, determination of microbial metabolites as surrogates for microbiome composition could offer practical biomarkers. Methods. Longitudinal fecal specimens (n = 451) were collected from 44 patients before HSCT through 100 days after transplantation, as well as 1-time samples from healthy volunteers (n = 18) as controls. Microbiota composition was determined using 16S ribosomal RNA V4 sequencing. Fecal indole and butyrate levels were determined using liquid chromatography tandem mass spectrometry. Results. Among HSCT recipients, both fecal indole and butyrate levels correlated with the Shannon diversity index at baseline (P = .02 and P = .002, respectively) and directly after transplantation (P = .006 and P < .001, respectively). Samples with high butyrate levels were enriched for Clostridiales, whereas samples containing high indole were also enriched for Bacteroidales. A lower Shannon diversity index at the time of engraftment was associated with increased incidence of acute intestinal graft-vs-host disease (iGVHD) (P = .02) and transplant-related deaths (P = .03). Although fecal metabolites were not associated with acute iGVHD or overall survival, patients contracting bloodstream infections within 30 days after transplantation had significantly lower levels of fecal butyrate (P = .03). Conclusions. Longitudinal analysis of fecal microbiome and metabolites after HSCT identified butyrate and indole as potential surrogate markers for microbial diversity and specific taxa. Further studies are needed to ascertain whether fecal metabolites can be used as biomarkers of acute iGVHD or bacteremia after HSCT.

Original languageEnglish (US)
JournalOpen Forum Infectious Diseases
Volume6
Issue number5
DOIs
StatePublished - Jan 1 2019

Fingerprint

Microbiota
Butyrates
Hematopoietic Stem Cells
Stem Cells
Transplants
Graft vs Host Disease
16S Ribosomal RNA
Transplantation
Biomarkers
RNA Sequence Analysis
Bacteremia
Tandem Mass Spectrometry
Liquid Chromatography
Healthy Volunteers
Survival
indole
Incidence
Infection

Keywords

  • Butyrate
  • Graft-vs-host disease (GVHD)
  • Hematopoietic stem cell transplant (HSCT)
  • Indole
  • Microbiome

ASJC Scopus subject areas

  • Oncology
  • Clinical Neurology

Cite this

Galloway-Peña, J. R., Peterson, C. B., Malik, F., Sahasrabhojane, P. V., Shah, D. P., Brumlow, C. E., ... Okhuysen, P. C. (2019). Fecal microbiome, metabolites, and stem cell transplant outcomes: A single-center pilot study. Open Forum Infectious Diseases, 6(5). https://doi.org/10.1093/ofid/ofz173

Fecal microbiome, metabolites, and stem cell transplant outcomes : A single-center pilot study. / Galloway-Peña, Jessica R.; Peterson, Christine B.; Malik, Farida; Sahasrabhojane, Pranoti V.; Shah, Dimpy P; Brumlow, Chelcy E.; Carlin, Lily G.; Chemaly, Roy F.; Im, Jin Seon; Rondon, Gabriela; Felix, Edd; Veillon, Lucas; Lorenzi, Philip L.; Alousi, Amin M.; Jenq, Robert R.; Kontoyiannis, Dimitrios P.; Shpall, Elizabeth J.; Shelburne, Samuel A.; Okhuysen, Pablo C.

In: Open Forum Infectious Diseases, Vol. 6, No. 5, 01.01.2019.

Research output: Contribution to journalArticle

Galloway-Peña, JR, Peterson, CB, Malik, F, Sahasrabhojane, PV, Shah, DP, Brumlow, CE, Carlin, LG, Chemaly, RF, Im, JS, Rondon, G, Felix, E, Veillon, L, Lorenzi, PL, Alousi, AM, Jenq, RR, Kontoyiannis, DP, Shpall, EJ, Shelburne, SA & Okhuysen, PC 2019, 'Fecal microbiome, metabolites, and stem cell transplant outcomes: A single-center pilot study', Open Forum Infectious Diseases, vol. 6, no. 5. https://doi.org/10.1093/ofid/ofz173
Galloway-Peña, Jessica R. ; Peterson, Christine B. ; Malik, Farida ; Sahasrabhojane, Pranoti V. ; Shah, Dimpy P ; Brumlow, Chelcy E. ; Carlin, Lily G. ; Chemaly, Roy F. ; Im, Jin Seon ; Rondon, Gabriela ; Felix, Edd ; Veillon, Lucas ; Lorenzi, Philip L. ; Alousi, Amin M. ; Jenq, Robert R. ; Kontoyiannis, Dimitrios P. ; Shpall, Elizabeth J. ; Shelburne, Samuel A. ; Okhuysen, Pablo C. / Fecal microbiome, metabolites, and stem cell transplant outcomes : A single-center pilot study. In: Open Forum Infectious Diseases. 2019 ; Vol. 6, No. 5.
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T1 - Fecal microbiome, metabolites, and stem cell transplant outcomes

T2 - A single-center pilot study

AU - Galloway-Peña, Jessica R.

AU - Peterson, Christine B.

AU - Malik, Farida

AU - Sahasrabhojane, Pranoti V.

AU - Shah, Dimpy P

AU - Brumlow, Chelcy E.

AU - Carlin, Lily G.

AU - Chemaly, Roy F.

AU - Im, Jin Seon

AU - Rondon, Gabriela

AU - Felix, Edd

AU - Veillon, Lucas

AU - Lorenzi, Philip L.

AU - Alousi, Amin M.

AU - Jenq, Robert R.

AU - Kontoyiannis, Dimitrios P.

AU - Shpall, Elizabeth J.

AU - Shelburne, Samuel A.

AU - Okhuysen, Pablo C.

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