Fasting regulates hypothalamic neuropeptide Y, agouti-related peptide, and proopiomelanocortin in diabetic mice independent of changes in leptin or insulin

T. M. Mizuno, H. Makimura, J. Silverstein, James Roberts, T. Lopingco, C. V. Mobbs

Research output: Contribution to journalArticle

158 Citations (Scopus)

Abstract

Fasting increases hypothalamic neuropeptide Y (NPY) and agoutirelated peptide (AGRP) messenger RNA (mRNA) and reduces hypothalamic POMC mRNA, and is also characterized by a reduction in plasma leptin, insulin, and glucose, each of which has been implicated in the regulation of hypothalamic gene expression. To further evaluate the roles of leptin, insulin, and glucose in mediating effects of fasting, we examined hypothalamic gene expression in nondiabetic and streptozotocin (STZ)-induced diabetic mice both under ad lib fed and 48-h fasted conditions. In both diabetic and nondiabetic mice, fasting stimulated hypothalamic NPY and AGRP mRNA and inhibited hypothalamic POMC mRNA and adipose leptin mRNA. However, in diabetic mice fasting had no effect on plasma leptin and insulin while decreasing plasma glucose, whereas in nondiabetic mice fasting decreased plasma leptin, insulin, and glucose. Furthermore, in non-diabetic fasted mice, NPY and AGRP mRNA were higher, and POMC mRNA and plasma glucose were lower, than in diabetic ad lib fed mice, even though insulin and leptin were similar in these two groups. These data are consistent with the hypothesis that although leptin and insulin regulate hypothalamic gene expression, glucose or other factors may have independent effects on hypothalamic and adipose gene expression under conditions of low insulin and leptin.

Original languageEnglish (US)
Pages (from-to)4551-4557
Number of pages7
JournalEndocrinology
Volume140
Issue number10
StatePublished - 1999
Externally publishedYes

Fingerprint

Pro-Opiomelanocortin
Neuropeptide Y
Leptin
Fasting
Insulin
Peptides
Messenger RNA
Glucose
Gene Expression
Dasyproctidae
Gene Expression Regulation
Streptozocin

ASJC Scopus subject areas

  • Endocrinology
  • Endocrinology, Diabetes and Metabolism

Cite this

Mizuno, T. M., Makimura, H., Silverstein, J., Roberts, J., Lopingco, T., & Mobbs, C. V. (1999). Fasting regulates hypothalamic neuropeptide Y, agouti-related peptide, and proopiomelanocortin in diabetic mice independent of changes in leptin or insulin. Endocrinology, 140(10), 4551-4557.

Fasting regulates hypothalamic neuropeptide Y, agouti-related peptide, and proopiomelanocortin in diabetic mice independent of changes in leptin or insulin. / Mizuno, T. M.; Makimura, H.; Silverstein, J.; Roberts, James; Lopingco, T.; Mobbs, C. V.

In: Endocrinology, Vol. 140, No. 10, 1999, p. 4551-4557.

Research output: Contribution to journalArticle

Mizuno, TM, Makimura, H, Silverstein, J, Roberts, J, Lopingco, T & Mobbs, CV 1999, 'Fasting regulates hypothalamic neuropeptide Y, agouti-related peptide, and proopiomelanocortin in diabetic mice independent of changes in leptin or insulin', Endocrinology, vol. 140, no. 10, pp. 4551-4557.
Mizuno, T. M. ; Makimura, H. ; Silverstein, J. ; Roberts, James ; Lopingco, T. ; Mobbs, C. V. / Fasting regulates hypothalamic neuropeptide Y, agouti-related peptide, and proopiomelanocortin in diabetic mice independent of changes in leptin or insulin. In: Endocrinology. 1999 ; Vol. 140, No. 10. pp. 4551-4557.
@article{c19d9ff6b2a34245983e913481794be9,
title = "Fasting regulates hypothalamic neuropeptide Y, agouti-related peptide, and proopiomelanocortin in diabetic mice independent of changes in leptin or insulin",
abstract = "Fasting increases hypothalamic neuropeptide Y (NPY) and agoutirelated peptide (AGRP) messenger RNA (mRNA) and reduces hypothalamic POMC mRNA, and is also characterized by a reduction in plasma leptin, insulin, and glucose, each of which has been implicated in the regulation of hypothalamic gene expression. To further evaluate the roles of leptin, insulin, and glucose in mediating effects of fasting, we examined hypothalamic gene expression in nondiabetic and streptozotocin (STZ)-induced diabetic mice both under ad lib fed and 48-h fasted conditions. In both diabetic and nondiabetic mice, fasting stimulated hypothalamic NPY and AGRP mRNA and inhibited hypothalamic POMC mRNA and adipose leptin mRNA. However, in diabetic mice fasting had no effect on plasma leptin and insulin while decreasing plasma glucose, whereas in nondiabetic mice fasting decreased plasma leptin, insulin, and glucose. Furthermore, in non-diabetic fasted mice, NPY and AGRP mRNA were higher, and POMC mRNA and plasma glucose were lower, than in diabetic ad lib fed mice, even though insulin and leptin were similar in these two groups. These data are consistent with the hypothesis that although leptin and insulin regulate hypothalamic gene expression, glucose or other factors may have independent effects on hypothalamic and adipose gene expression under conditions of low insulin and leptin.",
author = "Mizuno, {T. M.} and H. Makimura and J. Silverstein and James Roberts and T. Lopingco and Mobbs, {C. V.}",
year = "1999",
language = "English (US)",
volume = "140",
pages = "4551--4557",
journal = "Endocrinology",
issn = "0013-7227",
publisher = "The Endocrine Society",
number = "10",

}

TY - JOUR

T1 - Fasting regulates hypothalamic neuropeptide Y, agouti-related peptide, and proopiomelanocortin in diabetic mice independent of changes in leptin or insulin

AU - Mizuno, T. M.

AU - Makimura, H.

AU - Silverstein, J.

AU - Roberts, James

AU - Lopingco, T.

AU - Mobbs, C. V.

PY - 1999

Y1 - 1999

N2 - Fasting increases hypothalamic neuropeptide Y (NPY) and agoutirelated peptide (AGRP) messenger RNA (mRNA) and reduces hypothalamic POMC mRNA, and is also characterized by a reduction in plasma leptin, insulin, and glucose, each of which has been implicated in the regulation of hypothalamic gene expression. To further evaluate the roles of leptin, insulin, and glucose in mediating effects of fasting, we examined hypothalamic gene expression in nondiabetic and streptozotocin (STZ)-induced diabetic mice both under ad lib fed and 48-h fasted conditions. In both diabetic and nondiabetic mice, fasting stimulated hypothalamic NPY and AGRP mRNA and inhibited hypothalamic POMC mRNA and adipose leptin mRNA. However, in diabetic mice fasting had no effect on plasma leptin and insulin while decreasing plasma glucose, whereas in nondiabetic mice fasting decreased plasma leptin, insulin, and glucose. Furthermore, in non-diabetic fasted mice, NPY and AGRP mRNA were higher, and POMC mRNA and plasma glucose were lower, than in diabetic ad lib fed mice, even though insulin and leptin were similar in these two groups. These data are consistent with the hypothesis that although leptin and insulin regulate hypothalamic gene expression, glucose or other factors may have independent effects on hypothalamic and adipose gene expression under conditions of low insulin and leptin.

AB - Fasting increases hypothalamic neuropeptide Y (NPY) and agoutirelated peptide (AGRP) messenger RNA (mRNA) and reduces hypothalamic POMC mRNA, and is also characterized by a reduction in plasma leptin, insulin, and glucose, each of which has been implicated in the regulation of hypothalamic gene expression. To further evaluate the roles of leptin, insulin, and glucose in mediating effects of fasting, we examined hypothalamic gene expression in nondiabetic and streptozotocin (STZ)-induced diabetic mice both under ad lib fed and 48-h fasted conditions. In both diabetic and nondiabetic mice, fasting stimulated hypothalamic NPY and AGRP mRNA and inhibited hypothalamic POMC mRNA and adipose leptin mRNA. However, in diabetic mice fasting had no effect on plasma leptin and insulin while decreasing plasma glucose, whereas in nondiabetic mice fasting decreased plasma leptin, insulin, and glucose. Furthermore, in non-diabetic fasted mice, NPY and AGRP mRNA were higher, and POMC mRNA and plasma glucose were lower, than in diabetic ad lib fed mice, even though insulin and leptin were similar in these two groups. These data are consistent with the hypothesis that although leptin and insulin regulate hypothalamic gene expression, glucose or other factors may have independent effects on hypothalamic and adipose gene expression under conditions of low insulin and leptin.

UR - http://www.scopus.com/inward/record.url?scp=0033305272&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0033305272&partnerID=8YFLogxK

M3 - Article

C2 - 10499510

AN - SCOPUS:0033305272

VL - 140

SP - 4551

EP - 4557

JO - Endocrinology

JF - Endocrinology

SN - 0013-7227

IS - 10

ER -