Fast atom bombardment-mass spectrometric identification of molecular species of platelet-activating factor produced by stimulated human polymorphonuclear leukocytes

Susan T. Weintraub, Janet C. Ludwig, Glen E. Mott, Linda M. McManus, Cynthia Lear, R. Neal Pinckard

Research output: Contribution to journalArticle

40 Scopus citations

Abstract

Fast atom bombardment mass spectrometry was used to identify molecular species of platelet-activating factor (PAF) produced by stimulated human neutrophilic polymorphonuclear leukocytes. Normal and reverse-phase high performance liquid chromatography were employed to separate the individual regions with PAF activity prior to mass spectrometric analysis. The following alkyl chain homologs of acetyl glyceryl ether phosphorylcholine (AGEPC) were found: C16:0, C17:0, C18:0 and C18:1. There was also evidence for the presence of the C15:0 homolog, as well as other species which have not yet been identified.

Original languageEnglish (US)
Pages (from-to)868-876
Number of pages9
JournalBiochemical and Biophysical Research Communications
Volume129
Issue number3
DOIs
StatePublished - Jun 28 1985

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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