Fast atom bombardment-mass spectrometric identification of molecular species of platelet-activating factor produced by stimulated human polymorphonuclear leukocytes

Susan T. Weintraub; Janet C. Ludwig; Glen E. Mott; Linda M. McManus; Cynthia Lear; R. Neal Pinckard

doi:10.1016/0006-291X(85)91972-2

Fast atom bombardment-mass spectrometric identification of molecular species of platelet-activating factor produced by stimulated human polymorphonuclear leukocytes

Susan T. Weintraub, Janet C. Ludwig, Glen E. Mott, Linda M. McManus, Cynthia Lear, R. Neal Pinckard

Research output: Contribution to journal › Article › peer-review

44 Scopus citations

Abstract

Fast atom bombardment mass spectrometry was used to identify molecular species of platelet-activating factor (PAF) produced by stimulated human neutrophilic polymorphonuclear leukocytes. Normal and reverse-phase high performance liquid chromatography were employed to separate the individual regions with PAF activity prior to mass spectrometric analysis. The following alkyl chain homologs of acetyl glyceryl ether phosphorylcholine (AGEPC) were found: C16:0, C17:0, C18:0 and C18:1. There was also evidence for the presence of the C15:0 homolog, as well as other species which have not yet been identified.

Original language	English (US)
Pages (from-to)	868-876
Number of pages	9
Journal	Biochemical and Biophysical Research Communications
Volume	129
Issue number	3
DOIs	https://doi.org/10.1016/0006-291X(85)91972-2
State	Published - Jun 28 1985

ASJC Scopus subject areas

Molecular Biology
Biophysics
Biochemistry
Cell Biology

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10.1016/0006-291X(85)91972-2

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Weintraub, S. T., Ludwig, J. C., Mott, G. E., McManus, L. M., Lear, C., & Pinckard, R. N. (1985). Fast atom bombardment-mass spectrometric identification of molecular species of platelet-activating factor produced by stimulated human polymorphonuclear leukocytes. Biochemical and Biophysical Research Communications, 129(3), 868-876. https://doi.org/10.1016/0006-291X(85)91972-2

Fast atom bombardment-mass spectrometric identification of molecular species of platelet-activating factor produced by stimulated human polymorphonuclear leukocytes. / Weintraub, Susan T.; Ludwig, Janet C.; Mott, Glen E. et al.
In: Biochemical and Biophysical Research Communications, Vol. 129, No. 3, 28.06.1985, p. 868-876.

Research output: Contribution to journal › Article › peer-review

Weintraub, ST, Ludwig, JC, Mott, GE, McManus, LM, Lear, C & Pinckard, RN 1985, 'Fast atom bombardment-mass spectrometric identification of molecular species of platelet-activating factor produced by stimulated human polymorphonuclear leukocytes', Biochemical and Biophysical Research Communications, vol. 129, no. 3, pp. 868-876. https://doi.org/10.1016/0006-291X(85)91972-2

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title = "Fast atom bombardment-mass spectrometric identification of molecular species of platelet-activating factor produced by stimulated human polymorphonuclear leukocytes",

abstract = "Fast atom bombardment mass spectrometry was used to identify molecular species of platelet-activating factor (PAF) produced by stimulated human neutrophilic polymorphonuclear leukocytes. Normal and reverse-phase high performance liquid chromatography were employed to separate the individual regions with PAF activity prior to mass spectrometric analysis. The following alkyl chain homologs of acetyl glyceryl ether phosphorylcholine (AGEPC) were found: C16:0, C17:0, C18:0 and C18:1. There was also evidence for the presence of the C15:0 homolog, as well as other species which have not yet been identified.",

author = "Weintraub, {Susan T.} and Ludwig, {Janet C.} and Mott, {Glen E.} and McManus, {Linda M.} and Cynthia Lear and Pinckard, {R. Neal}",

note = "Funding Information: We thank Richard technical assistance study was supported Castillo, Patricia Padilla and Kelly Walton for and Laida Garcia for the typing of this manuscript. in part by NIB Grants BL 22555 and AI 21818.",

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AU - Mott, Glen E.

AU - McManus, Linda M.

AU - Lear, Cynthia

AU - Pinckard, R. Neal

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Fast atom bombardment-mass spectrometric identification of molecular species of platelet-activating factor produced by stimulated human polymorphonuclear leukocytes

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