Fas-mediated apoptosis in a rat acinar cell line is dependent on caspase-1 activity

Sonomi Aiba-Masago, Rejei Masago, Norma Vela-Roch, Norman Talal, Howard Dang

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Apoptosis plays an important role in the dysfunction of exocrine glands. Fas is a death-inducing receptor found on many types of cells including epithelial acinar cells. To elucidate the intracellular mechanism of Fas-mediated cell death in exocrine glands, an epithelial acinar cell line, SMG-C6, was studied. Caspase-1, -3, -8, and -9 activities were elevated in SMG-C6 cells after the induction of apoptosis by soluble Fas ligand (FasL). The activation of caspase-1 and -8 occurred prior to caspase-3 and -9 activation. The caspase-1 inhibitor, zYVAD-fmk, was effective in preventing cell death, whereas the caspase-3 and -8 inhibitors (ac-DEVD-CHO and ac-IETD-CHO, respectively) were not. zYVAD-fmk was able to inhibit caspase-3 activation indicating that caspase-1 is upstream to caspase-3. Furthermore, kinetic studies show that caspase-1 is an early event in the Fas apoptotic pathway. This study shows that caspase-1 participates in Fas-mediated apoptosis of epithelial cells by initiating the caspase cascade.

Original languageEnglish (US)
Pages (from-to)617-624
Number of pages8
JournalCellular Signalling
Volume13
Issue number9
DOIs
StatePublished - 2001

Fingerprint

Caspase 1
Acinar Cells
Caspase 3
Apoptosis
Cell Line
Exocrine Glands
Caspase 8
Epithelial Cells
Cell Death
Death Domain Receptors
Fas Ligand Protein
Caspase 9
Caspases

Keywords

  • Apoptosis
  • CD95 caspase
  • Epithelial cells
  • Salivary gland
  • Sjögren's syndrome

ASJC Scopus subject areas

  • Cell Biology

Cite this

Fas-mediated apoptosis in a rat acinar cell line is dependent on caspase-1 activity. / Aiba-Masago, Sonomi; Masago, Rejei; Vela-Roch, Norma; Talal, Norman; Dang, Howard.

In: Cellular Signalling, Vol. 13, No. 9, 2001, p. 617-624.

Research output: Contribution to journalArticle

Aiba-Masago, S, Masago, R, Vela-Roch, N, Talal, N & Dang, H 2001, 'Fas-mediated apoptosis in a rat acinar cell line is dependent on caspase-1 activity', Cellular Signalling, vol. 13, no. 9, pp. 617-624. https://doi.org/10.1016/S0898-6568(01)00183-8
Aiba-Masago, Sonomi ; Masago, Rejei ; Vela-Roch, Norma ; Talal, Norman ; Dang, Howard. / Fas-mediated apoptosis in a rat acinar cell line is dependent on caspase-1 activity. In: Cellular Signalling. 2001 ; Vol. 13, No. 9. pp. 617-624.
@article{b1e0f1abef124c43befe73e8681e8787,
title = "Fas-mediated apoptosis in a rat acinar cell line is dependent on caspase-1 activity",
abstract = "Apoptosis plays an important role in the dysfunction of exocrine glands. Fas is a death-inducing receptor found on many types of cells including epithelial acinar cells. To elucidate the intracellular mechanism of Fas-mediated cell death in exocrine glands, an epithelial acinar cell line, SMG-C6, was studied. Caspase-1, -3, -8, and -9 activities were elevated in SMG-C6 cells after the induction of apoptosis by soluble Fas ligand (FasL). The activation of caspase-1 and -8 occurred prior to caspase-3 and -9 activation. The caspase-1 inhibitor, zYVAD-fmk, was effective in preventing cell death, whereas the caspase-3 and -8 inhibitors (ac-DEVD-CHO and ac-IETD-CHO, respectively) were not. zYVAD-fmk was able to inhibit caspase-3 activation indicating that caspase-1 is upstream to caspase-3. Furthermore, kinetic studies show that caspase-1 is an early event in the Fas apoptotic pathway. This study shows that caspase-1 participates in Fas-mediated apoptosis of epithelial cells by initiating the caspase cascade.",
keywords = "Apoptosis, CD95 caspase, Epithelial cells, Salivary gland, Sj{\"o}gren's syndrome",
author = "Sonomi Aiba-Masago and Rejei Masago and Norma Vela-Roch and Norman Talal and Howard Dang",
year = "2001",
doi = "10.1016/S0898-6568(01)00183-8",
language = "English (US)",
volume = "13",
pages = "617--624",
journal = "Cellular Signalling",
issn = "0898-6568",
publisher = "Elsevier Inc.",
number = "9",

}

TY - JOUR

T1 - Fas-mediated apoptosis in a rat acinar cell line is dependent on caspase-1 activity

AU - Aiba-Masago, Sonomi

AU - Masago, Rejei

AU - Vela-Roch, Norma

AU - Talal, Norman

AU - Dang, Howard

PY - 2001

Y1 - 2001

N2 - Apoptosis plays an important role in the dysfunction of exocrine glands. Fas is a death-inducing receptor found on many types of cells including epithelial acinar cells. To elucidate the intracellular mechanism of Fas-mediated cell death in exocrine glands, an epithelial acinar cell line, SMG-C6, was studied. Caspase-1, -3, -8, and -9 activities were elevated in SMG-C6 cells after the induction of apoptosis by soluble Fas ligand (FasL). The activation of caspase-1 and -8 occurred prior to caspase-3 and -9 activation. The caspase-1 inhibitor, zYVAD-fmk, was effective in preventing cell death, whereas the caspase-3 and -8 inhibitors (ac-DEVD-CHO and ac-IETD-CHO, respectively) were not. zYVAD-fmk was able to inhibit caspase-3 activation indicating that caspase-1 is upstream to caspase-3. Furthermore, kinetic studies show that caspase-1 is an early event in the Fas apoptotic pathway. This study shows that caspase-1 participates in Fas-mediated apoptosis of epithelial cells by initiating the caspase cascade.

AB - Apoptosis plays an important role in the dysfunction of exocrine glands. Fas is a death-inducing receptor found on many types of cells including epithelial acinar cells. To elucidate the intracellular mechanism of Fas-mediated cell death in exocrine glands, an epithelial acinar cell line, SMG-C6, was studied. Caspase-1, -3, -8, and -9 activities were elevated in SMG-C6 cells after the induction of apoptosis by soluble Fas ligand (FasL). The activation of caspase-1 and -8 occurred prior to caspase-3 and -9 activation. The caspase-1 inhibitor, zYVAD-fmk, was effective in preventing cell death, whereas the caspase-3 and -8 inhibitors (ac-DEVD-CHO and ac-IETD-CHO, respectively) were not. zYVAD-fmk was able to inhibit caspase-3 activation indicating that caspase-1 is upstream to caspase-3. Furthermore, kinetic studies show that caspase-1 is an early event in the Fas apoptotic pathway. This study shows that caspase-1 participates in Fas-mediated apoptosis of epithelial cells by initiating the caspase cascade.

KW - Apoptosis

KW - CD95 caspase

KW - Epithelial cells

KW - Salivary gland

KW - Sjögren's syndrome

UR - http://www.scopus.com/inward/record.url?scp=0034902994&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0034902994&partnerID=8YFLogxK

U2 - 10.1016/S0898-6568(01)00183-8

DO - 10.1016/S0898-6568(01)00183-8

M3 - Article

C2 - 11495719

AN - SCOPUS:0034902994

VL - 13

SP - 617

EP - 624

JO - Cellular Signalling

JF - Cellular Signalling

SN - 0898-6568

IS - 9

ER -