TY - JOUR
T1 - Farnesyl transferase inhibitor (R115777)-induced inhibition of STAT3 (Tyr705) phosphorylation in human pancreatic cancer cell lines require extracellular signal-regulated kinases
AU - Venkatasubbarao, Kolaparthi
AU - Choudary, Ahsan
AU - Freeman, James W.
N1 - Copyright:
Copyright 2008 Elsevier B.V., All rights reserved.
PY - 2005/4/1
Y1 - 2005/4/1
N2 - In this study, we report that R115777, a nonpeptidomimetic farnesyl transferase inhibitor, suppresses the growth of human pancreatic adenocarcinoma cell lines and that this growth inhibition is associated with modulation in the phosphorylation levels of signal transducers and activators of transcription 3 (STAT3) and extracellular signal-regulated kinases (ERK). Treatment of cells with R115777 inhibited the tyrosine phosphorylation of STAT3(Tyr705), while increasing the serine phosphorylation of STAT3(Ser727). We found the differential phosphorylation of STAT3 was due to an increased and prolonged activation of ERKs. The biological significance of ERK-mediated inhibition of STAT3(Tyr705) phosphorylation was further assessed by treating the cells with an inhibitor (PD98059) of mitogen-activated protein kinase kinase (MEK) or by transfecting the cells with a vector that expresses constitatively active MEK-1. Expression of constitutively active MEK-1 caused an increase of ERK activity and inhibited STAT3(Tyr705) phosphorylation. Conversely, inhibition of ERK activity by PD98059 reversed the R115777-induced inhibition of STAT3(Tyr705) phosphorylation. R115777 also caused the inhibition of the binding of STAT3 to its consensus binding element. An increase in the activation of ERKs either by overexpressing MEK-1 or treatment of cells with R115777 caused an up-regulation in the levels of a cyclin-dependent kinase (cdk) inhibitor, p21cip1/waf1. These observations suggest that R115777-induced growth inhibition is partly due to the prolonged activation of ERKs that mediates an inhibition of STAT3 (Tyr705) phosphorylation and an increase in the levels of p21 cip1/waf1 in human pancreatic adenocarcinoma cell lines.
AB - In this study, we report that R115777, a nonpeptidomimetic farnesyl transferase inhibitor, suppresses the growth of human pancreatic adenocarcinoma cell lines and that this growth inhibition is associated with modulation in the phosphorylation levels of signal transducers and activators of transcription 3 (STAT3) and extracellular signal-regulated kinases (ERK). Treatment of cells with R115777 inhibited the tyrosine phosphorylation of STAT3(Tyr705), while increasing the serine phosphorylation of STAT3(Ser727). We found the differential phosphorylation of STAT3 was due to an increased and prolonged activation of ERKs. The biological significance of ERK-mediated inhibition of STAT3(Tyr705) phosphorylation was further assessed by treating the cells with an inhibitor (PD98059) of mitogen-activated protein kinase kinase (MEK) or by transfecting the cells with a vector that expresses constitatively active MEK-1. Expression of constitutively active MEK-1 caused an increase of ERK activity and inhibited STAT3(Tyr705) phosphorylation. Conversely, inhibition of ERK activity by PD98059 reversed the R115777-induced inhibition of STAT3(Tyr705) phosphorylation. R115777 also caused the inhibition of the binding of STAT3 to its consensus binding element. An increase in the activation of ERKs either by overexpressing MEK-1 or treatment of cells with R115777 caused an up-regulation in the levels of a cyclin-dependent kinase (cdk) inhibitor, p21cip1/waf1. These observations suggest that R115777-induced growth inhibition is partly due to the prolonged activation of ERKs that mediates an inhibition of STAT3 (Tyr705) phosphorylation and an increase in the levels of p21 cip1/waf1 in human pancreatic adenocarcinoma cell lines.
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U2 - 10.1158/0008-5472.CAN-04-2396
DO - 10.1158/0008-5472.CAN-04-2396
M3 - Article
C2 - 15805288
AN - SCOPUS:16844365486
VL - 65
SP - 2861
EP - 2871
JO - Cancer Research
JF - Cancer Research
SN - 0008-5472
IS - 7
ER -