Factors (TAFs) required for activated transcription interact with TATA box-binding protein conserved core domain

Qiang Zhou, Thomas G. Boyer, Arnold J. Berk

Research output: Contribution to journalArticlepeer-review

64 Scopus citations

Abstract

TFIID is a multisubunit protein containing the TATA box-binding polypeptide (TBP) and associated factors (TFIID-TAFs) required for activated transcription by RNA polymerase II. TBPs from different eukaryotes contain a highly conserved carboxy-terminal domain and very divergent amino-terminal domains. Earlier studies proposed that the amino-terminal domains of metazoan TBPs are required for activated transcription. However, we report that a human TFIID complex containing an amino-terminal truncated TBP contains all the major TFIID-TAFs and supports in vitro transcriptional stimulation by different classes of activation domains and from a TATA-less promoter. Protein blotting experiments revealed direct interactions between the conserved domain of TBP and the two largest TAFs. The results suggest a model for the interaction of TFIID-TAFs with TBP.

Original languageEnglish (US)
Pages (from-to)180-187
Number of pages8
JournalGenes and Development
Volume7
Issue number2
DOIs
StatePublished - 1993

Keywords

  • RNA polymerase II
  • TBP-associated factors
  • TFIID
  • Transcription factor
  • Transcriptional activation

ASJC Scopus subject areas

  • Genetics
  • Developmental Biology

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