Facile generation of heat-stable antiviral and antitoxin single domain antibodies from a semisynthetic llama library

Ellen R. Goldman, George P. Andersen, Jinny L. Liu, James B. Delehanty, Laura J. Sherwood, Lisa E. Osborn, Larry B. Cummins, Andrew Hayhurst

    Research output: Contribution to journalArticle

    138 Scopus citations

    Abstract

    Llamas possess a class of unconventional immunoglobulins that have only heavy chains; unpaired heavy variable domains are responsible for antigen binding. These domains have previously been cloned and expressed as single domain antibodies (sdAbs); they comprise the smallest known antigen binding fragments. SdAbs have been shown to bind antigens at > 90 °C and to refold after being denatured. To take advantage of the remarkable properties of sdAbs, we constructed a large, semisynthetic llama sdAb library. This library facilitated the rapid selection of binders to an array of biothreat targets. We selected sdAb specific for live vaccinia virus (a smallpox virus surrogate), hen egg lysozyme, cholera toxin, ricin, and staphylococcal enterotoxin B. The selected sdAb possessed high specificity as well as enhanced thermal stability in comparison to conventional IgG and scFv antibodies. We also determined equilibrium dissociation constants as well as demonstrated the use of several antitoxin sdAbs as effective capture and reporter molecules in sandwich assays on the Luminex instrument. The ability to rapidly select such rugged antibodies will enhance the reliability of immunoassays by extending shelf life and the capacity to function in hostile environments.

    Original languageEnglish (US)
    Pages (from-to)8245-8255
    Number of pages11
    JournalAnalytical Chemistry
    Volume78
    Issue number24
    DOIs
    StatePublished - Dec 15 2006

    ASJC Scopus subject areas

    • Analytical Chemistry

    Fingerprint Dive into the research topics of 'Facile generation of heat-stable antiviral and antitoxin single domain antibodies from a semisynthetic llama library'. Together they form a unique fingerprint.

  • Cite this

    Goldman, E. R., Andersen, G. P., Liu, J. L., Delehanty, J. B., Sherwood, L. J., Osborn, L. E., Cummins, L. B., & Hayhurst, A. (2006). Facile generation of heat-stable antiviral and antitoxin single domain antibodies from a semisynthetic llama library. Analytical Chemistry, 78(24), 8245-8255. https://doi.org/10.1021/ac0610053