Förster resonance energy transfer-based imaging at the cell surface of live cells

Sonya M. Bierbower, Mark S. Shapiro

Research output: Chapter in Book/Report/Conference proceedingChapter

2 Scopus citations

Abstract

Understanding the molecular mechanisms of protein-protein interactions at the cell surface of living cells is fundamental to identifying the nature of cellular processes. Here, we discuss how fluorescence-based approaches have been successfully developed to visualize protein-protein interactions in living cells. Förster resonance energy transfer (FRET) is unique in generating fluorescence signals between proteins that are highly spatially sensitive. Furthermore, total internal reflectance fluorescence (TIRF) microscopy combined with FRET is a robust technique used to assay protein/protein interactions and the functionality of proteins assembled at the cell surface membrane.

Original languageEnglish (US)
Title of host publicationIon Channels
Subtitle of host publicationMethods and Protocols
EditorsNikita Gamper
Pages209-216
Number of pages8
DOIs
StatePublished - 2013

Publication series

NameMethods in Molecular Biology
Volume998
ISSN (Print)1064-3745

Keywords

  • Cell surface
  • FRET
  • Live-cell imaging
  • Protein-protein interactions
  • TIRF

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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  • Cite this

    Bierbower, S. M., & Shapiro, M. S. (2013). Förster resonance energy transfer-based imaging at the cell surface of live cells. In N. Gamper (Ed.), Ion Channels: Methods and Protocols (pp. 209-216). (Methods in Molecular Biology; Vol. 998). https://doi.org/10.1007/978-1-62703-351-0_16