@inbook{1af50c8a2aeb4eeeaba820a750623571,
title = "F{\"o}rster resonance energy transfer-based imaging at the cell surface of live cells",
abstract = "Understanding the molecular mechanisms of protein-protein interactions at the cell surface of living cells is fundamental to identifying the nature of cellular processes. Here, we discuss how fluorescence-based approaches have been successfully developed to visualize protein-protein interactions in living cells. F{\"o}rster resonance energy transfer (FRET) is unique in generating fluorescence signals between proteins that are highly spatially sensitive. Furthermore, total internal reflectance fluorescence (TIRF) microscopy combined with FRET is a robust technique used to assay protein/protein interactions and the functionality of proteins assembled at the cell surface membrane.",
keywords = "Cell surface, FRET, Live-cell imaging, Protein-protein interactions, TIRF",
author = "Bierbower, {Sonya M.} and Shapiro, {Mark S.}",
year = "2013",
doi = "10.1007/978-1-62703-351-0_16",
language = "English (US)",
isbn = "9781627033503",
series = "Methods in Molecular Biology",
pages = "209--216",
editor = "Nikita Gamper",
booktitle = "Ion Channels",
}