Extramitochondrial localization of mortalin/mthsp70/PBP74/GRP75

Qitao Ran, Renu Wadhwa, Rena Kawai, Sunil C. Kaul, Richard N. Sifers, Roger J. Bick, James R. Smith, Olivia M. Pereira-Smith

Research output: Contribution to journalArticlepeer-review

137 Scopus citations

Abstract

Subcellular fractionation and immunofluorescence microscopy were used to identify the specific sites of intracellular residence of mortalin, also called a mitochondrial homologue of the hsp70 family, in immortal human cell lines previously assigned to four distinct complementation groups (A-D) for indefinite cell division. In addition to the mitochondria it was seen in the endoplasmic reticulum (ER) fractions of all the cell lines analyzed. Interestingly, three of the group A cells lines (EJ, GM639, and HT1080), in addition to the mitochondria and ER, exhibited cytosolically (extra-organelle) localized pool of mortalin. These findings demonstrate that mortalin is not present exclusively in mitochondria. Its residence in different organelles may be the basis of differential distribution observed previously in different human cell lines. (C) 2000 Academic Press.

Original languageEnglish (US)
Pages (from-to)174-179
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume275
Issue number1
DOIs
StatePublished - Aug 18 2000
Externally publishedYes

Keywords

  • Cell immortalization
  • Intracellular localization
  • Mortalin

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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