Extraction and quantification of epibatidine in plasma

M. A. Javors, J. J. Sanchez, T. S. King, A. R. Rohde, S. G. Wilson, C. M. Flores

Research output: Contribution to journalArticle

2 Scopus citations

Abstract

Epibatidine was extracted from human and mouse plasma into a hexane-isopropanol mixture and back-extracted into a phosphate buffer, pH 2.5, then identified by HPLC isocratically using a CN column and quantified with ultraviolet detection at a fixed wavelength of 214 nm. The percent recovery of epibatidine from spiked plasma samples was 83.6% and the percent extraction was linear between 10 and 1000 ng/ml. Desipramine was used as the internal standard. For spiked control samples containing 50 and 750 ng/ml, between-day precisions were 20.8 and 7.2% (RSD%), respectively; accuracy was 87.0 and 99.1%, respectively. The limit of detection was 2 ng/ml. Using this method, an intraperitoneal dose of 0.1 mg/kg of epibatidine produced mean levels of 7.3 and 37.1 ng/ml in pooled male and female plasma samples from C57BL/10 J mice, respectively. This is a simple and straightforward procedure by which plasma samples may be analyzed for epibatidine.

Original languageEnglish (US)
Pages (from-to)379-382
Number of pages4
JournalJournal of Chromatography B: Biomedical Sciences and Applications
Volume755
Issue number1-2
DOIs
StatePublished - May 5 2001

Keywords

  • Epibatidine

ASJC Scopus subject areas

  • Chemistry(all)

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