Extrachromosomal human immunodeficiency virus type-1 DNA can initiate a spreading infection of HL-60 cells

S. T. Butera, V. L. Perez, N. J. Besansky, W. C. Chan, B. Y. Wu, G. J. Nabel, T. M. Folks

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

In this report, we describe a human immunodeficiency virus type-1 (HIV-1)-infected promyelocytic cell line, OM, derived from HL-60 cellls. Although the OM cell line was biologically cloned twice, the pattern of HIV-1 expression during culture appeared analogous to a classical acute spreading infection and was inhibited by both azidothymidine and recombinant soluble CD4 treatment. The number of OM cells actually expressing HIV-1 at the beginning of culture was 0%, reached a peak of nearly 100% at 6 weeks, and then fell to <10% HIV-1+ cells by 10 weeks. Clonal analysis of the surviving cells verified that stable HIV-1+ OM cells resulted from the spreading infection. Southern analysis confirmed the transmission of HIV-1 through these OM cultures and the occurrence of stable clones which resulted. The initial percentage of OM cells actually harboring the HIV-1 genome was <0.1%, indicating nonfaithful transmission of an unintegrated HIV-1 genome during clonal expansion. These results demonstrate that extrachromosomal HIV-1 DNA can contribute to the spread of HIV-1 infection and give rise to cells which have stably integrated HIV-1 provirus.

Original languageEnglish (US)
Pages (from-to)366-373
Number of pages8
JournalJournal of Cellular Biochemistry
Volume45
Issue number4
StatePublished - 1991
Externally publishedYes

Fingerprint

HL-60 Cells
Viruses
HIV-1
DNA
Infection
Genes
Cells
Genome
Cell Line
Proviruses
Zidovudine
Virus Diseases
Clone Cells
Cell Count

Keywords

  • Azidothymidine
  • CD4
  • Clonal analysis
  • HIV-1 infection of promyelocytes
  • Unintegrated HIV-1 DNA

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology

Cite this

Butera, S. T., Perez, V. L., Besansky, N. J., Chan, W. C., Wu, B. Y., Nabel, G. J., & Folks, T. M. (1991). Extrachromosomal human immunodeficiency virus type-1 DNA can initiate a spreading infection of HL-60 cells. Journal of Cellular Biochemistry, 45(4), 366-373.

Extrachromosomal human immunodeficiency virus type-1 DNA can initiate a spreading infection of HL-60 cells. / Butera, S. T.; Perez, V. L.; Besansky, N. J.; Chan, W. C.; Wu, B. Y.; Nabel, G. J.; Folks, T. M.

In: Journal of Cellular Biochemistry, Vol. 45, No. 4, 1991, p. 366-373.

Research output: Contribution to journalArticle

Butera, ST, Perez, VL, Besansky, NJ, Chan, WC, Wu, BY, Nabel, GJ & Folks, TM 1991, 'Extrachromosomal human immunodeficiency virus type-1 DNA can initiate a spreading infection of HL-60 cells', Journal of Cellular Biochemistry, vol. 45, no. 4, pp. 366-373.
Butera, S. T. ; Perez, V. L. ; Besansky, N. J. ; Chan, W. C. ; Wu, B. Y. ; Nabel, G. J. ; Folks, T. M. / Extrachromosomal human immunodeficiency virus type-1 DNA can initiate a spreading infection of HL-60 cells. In: Journal of Cellular Biochemistry. 1991 ; Vol. 45, No. 4. pp. 366-373.
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