Extinction of liver/bone/kidney alkaline phosphatase in osteosarcoma hybrid cells

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3 Scopus citations


We have constructed a series of interspecific somatic cell hybrids between the human osteoblast-like osteosarcoma, TE85, and a mouse fibrosarcoma, La-t-. In these whole-cell hybrids, we observed a 10-fold reduction of human liver/bone/kidney (L/B/K) alkaline phosphatase steady-state mRNA and alkaline phosphatase protein activity. The phenomenon of loss of tissue-specific gene expression has been termed extinction. Subclones of these hybrids were isolated, which reexpressed the alkaline phosphatase gene product. These late-passage hybrids had a reduced number of mouse fibroblast chromosomes when compared to earlier passages. This suggests that a trans-acting negative regulatory element, encoded in the fibroblast genome, regulates expression of L/B/K alkaline phosphatase. This is the first evidence that extinction plays a role in the regulation of osteoblast gene expression.

Original languageEnglish (US)
Pages (from-to)423-430
Number of pages8
JournalSomatic Cell and Molecular Genetics
Issue number5
StatePublished - Sep 1992

ASJC Scopus subject areas

  • Genetics
  • Cell Biology


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