External peer review quality assurance testing in von Willebrand disease: The recent experience of the United States College of American Pathologists proficiency testing program

Timothy E. Hayes, John T. Brandt, Wayne L. Chandler, Charles S. Eby, Kandice Kottke-Marchant, Jayashree Krishnan, Jerry B. Lefkowitz, John D. Olson, Chad R. Rund, Elizabeth M. Van Cott, Mark T. Cunningham

Research output: Contribution to journalArticle

38 Scopus citations

Abstract

The U.S. College of American Pathologists (CAP) has conducted a focused study of the proficiency testing for von Willebrand disease (vWD) analysis from 2003 to 2005. This report summarizes the findings regarding the accuracy and precision of the various assays at different analyte levels, as well as the influence of the reference material used to construct the assay standard curve. The results show that testing of von Willebrand factor (vWF):antigen (vWF:Ag) and ristocetin cofactor activity (vWF:RCo) is reasonably accurate, with all-method mean values falling within 3.2 and 5.6%, respectively, of the International Society on Thrombosis and Haemostasis Secondary Coagulation Standard (lot 2) assigned values. vWF:Ag measurements are reasonably precise (all-method coefficients of variation [CVs] = 10.7 to 15.1%), even at lower levels of vWF. The highest precision was observed for immunoturbidometric assays (CVs, 6.3 to 9.7%). vWF:RCo measurements are less precise (all-method CVs, 23.3 to 30.9%). The reference materials used in the standard curves for immunoturbidometric vWF:Ag assays appear to have accurately assigned vWF values for the majority of commercial suppliers.

Original languageEnglish (US)
Pages (from-to)499-504
Number of pages6
JournalSeminars in Thrombosis and Hemostasis
Volume32
Issue number5
DOIs
StatePublished - Jul 2006

Keywords

  • Hemostasis testing
  • Proficiency testing
  • Quality assurance
  • Quality control
  • Von Willebrand disease (vWD)
  • Von Willebrand factor (vWF)

ASJC Scopus subject areas

  • Hematology
  • Cardiology and Cardiovascular Medicine

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    Hayes, T. E., Brandt, J. T., Chandler, W. L., Eby, C. S., Kottke-Marchant, K., Krishnan, J., Lefkowitz, J. B., Olson, J. D., Rund, C. R., Van Cott, E. M., & Cunningham, M. T. (2006). External peer review quality assurance testing in von Willebrand disease: The recent experience of the United States College of American Pathologists proficiency testing program. Seminars in Thrombosis and Hemostasis, 32(5), 499-504. https://doi.org/10.1055/s-2006-947864