Expression of UGA-containing Mycoplasma genes in Bacillus subtilis

T. R. Kannan, Joel B. Baseman

Research output: Contribution to journalArticlepeer-review

7 Scopus citations

Abstract

We used Bacillus subtilis to express UGA-containing Mycoplasma genes encoding the P30 adhesin (one UGA) of Mycoplasma pneumoniae and methionine sulfoxide reductase (two UGAs) of Mycoplasma genitalium. Due to natural UGA suppression, these Mycoplasma genes were expressed as full-length protein products, but at relatively low efficiency, in recombinant wild-type Bacillus. The B. subtilis-expressed Mycoplasma proteins appeared as single bands and not as multiple bands compared to expression in recombinant Escherichia coli. Bacillus mutants carrying mutations in the structural gene (prfB) for release factor 2 markedly enhanced the level of readthrough of UGA-containing Mycoplasma genes.

Original languageEnglish (US)
Pages (from-to)2664-2667
Number of pages4
JournalJournal of bacteriology
Volume182
Issue number9
DOIs
StatePublished - May 2000

ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology

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