Expression of the la crosse m segment proteins in a recombinant vaccinia expression system mediates ph-dependent cellular fusion

David R. Jacoby, Charles Cooke, Indira Prabakaran, Jocelyn Boland, Neal Nathanson, Francisco Gonzalez-Scarano

Research output: Contribution to journalArticlepeer-review

29 Scopus citations

Abstract

To study the expression of La Crosse virus (LAC) glycaproteins, G1 and G2, we constructed a cDNA copy of the open reading frame (ORF) of the middle RNA segment and expressed it in a recombinant vaccinia virus (VV.ORF). Cells infected with W.ORF expressed G1 and G2 at the cell surface and formed syncytia with a pH profile similar to that of LAC. These experiments provide a system of studying the biological functions of the LAC glycoproteins, including processing, targeting, fusion, receptor binding, and antigenicity.

Original languageEnglish (US)
Pages (from-to)993-996
Number of pages4
JournalVirology
Volume193
Issue number2
DOIs
StatePublished - Apr 1993

ASJC Scopus subject areas

  • Virology

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