Expression of the interleukin-18 gene from rhesus macaque by the simian immunodeficiency virus does not result in increased viral replication

L. D. Giavedoni, J. D. Imhoof, M. C. Velasquillo, L. M. Parodi, V. L. Hodara

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Interleukin-18 (IL-18), previously known as interferon-γ (IFN-γ)-inducing factor (IGIF), is a proinflammatory cytokine expressed by activated macrophages that acts in synergy with IL-12 as an important amplifying factor for IFN-γ production and Th1 development. To study the effect of IL-18 on a lentiviral infection, we cloned the IL-18 gene from a rhesus macaque and constructed replication-competent simian immunodeficiency virus (SIV) that expressed either the precursor pro-IL-18 (SIVIL-18) or the mature form (SIVmIL-18) of IL-18. The predicted amino acid sequence for rhesus IL-18 had 96% homology with the human one, differing in only 8 of 193 residues. SIVIL-18 and SIVmIL-18 replicated more slowly than control viruses in the CEM × 3174 cell line and resulted in the development of chronically infected cell lines that expressed high levels of infectious SIV. The cell line generated by SIVIL-18 released large quantities of IL-18 into the supernatant, whereas the one obtained from SIVmIL-18 showed the accumulation of IL-18 in the cytoplasm. Similarly, SIVIL-18 and SIVmIL-18 replicated more slowly than the unmodified viral vector in rhesus peripheral blood mononuclear cells (PMBC), but only SIVIL-18 expressed biologically active IL-18. These experiments show that the precursor form of IL-18 is necessary for the efficient release of the cytokine and that IL-18 does not promote increased replication of SIV in rhesus PBMC.

Original languageEnglish (US)
Pages (from-to)173-180
Number of pages8
JournalJournal of Interferon and Cytokine Research
Volume21
Issue number3
DOIs
StatePublished - 2001
Externally publishedYes

Fingerprint

Simian Immunodeficiency Virus
Interleukin-18
Macaca mulatta
Genes
Cell Line
Interferons
Cytokines
Interleukin-12
Amino Acid Sequence
Blood Cells
Cytoplasm
Macrophages

ASJC Scopus subject areas

  • Immunology
  • Virology
  • Cell Biology

Cite this

Expression of the interleukin-18 gene from rhesus macaque by the simian immunodeficiency virus does not result in increased viral replication. / Giavedoni, L. D.; Imhoof, J. D.; Velasquillo, M. C.; Parodi, L. M.; Hodara, V. L.

In: Journal of Interferon and Cytokine Research, Vol. 21, No. 3, 2001, p. 173-180.

Research output: Contribution to journalArticle

Giavedoni, L. D. ; Imhoof, J. D. ; Velasquillo, M. C. ; Parodi, L. M. ; Hodara, V. L. / Expression of the interleukin-18 gene from rhesus macaque by the simian immunodeficiency virus does not result in increased viral replication. In: Journal of Interferon and Cytokine Research. 2001 ; Vol. 21, No. 3. pp. 173-180.
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