Expression of the dominant-negative regulator Id4 is induced during adipocyte differentiation

Heping Chen; Yi Chinn Weng; Gina C. Schatteman; Laura Sanders; Robert J. Christy; Barbara A. Christy

doi:10.1006/bbrc.1999.0386

Expression of the dominant-negative regulator Id4 is induced during adipocyte differentiation

Heping Chen, Yi Chinn Weng, Gina C. Schatteman, Laura Sanders, Robert J. Christy, Barbara A. Christy

Research output: Contribution to journal › Article › peer-review

22 Scopus citations

Abstract

Expression of the Id4 gene was investigated during differentiation of 3T3-L1 preadipocytes into mature adipocytes. Id4 is a member of a family encoding non-DNA binding helix-loop-helix proteins proposed to inhibit the activity of basic HLH (bHLH) proteins important in many developmental processes. We show here that Id4 expression is low in confluent preadipocytes and rapidly induced by treatment with the combination of hormones which causes differentiation into mature adipocytes. Id4 expression is also induced by treatment with individual hormones, especially dexamathasone. Id4 mRNA can be detected in mouse and human adipose tissue. Genes encoding E-proteins (bHLH proteins known to interact with and be regulated by Id proteins) are expressed and regulated during differentiation in 3T3-L1 cells. These data suggest that the Id4 transcriptional regulator is playing a role in adipose cell differentiation and suggest that DNA-binding HLH proteins may also be important in regulation of differentiation of these cells.

Original language	English (US)
Pages (from-to)	614-619
Number of pages	6
Journal	Biochemical and Biophysical Research Communications
Volume	256
Issue number	3
DOIs	https://doi.org/10.1006/bbrc.1999.0386
State	Published - Mar 24 1999

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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title = "Expression of the dominant-negative regulator Id4 is induced during adipocyte differentiation",

abstract = "Expression of the Id4 gene was investigated during differentiation of 3T3-L1 preadipocytes into mature adipocytes. Id4 is a member of a family encoding non-DNA binding helix-loop-helix proteins proposed to inhibit the activity of basic HLH (bHLH) proteins important in many developmental processes. We show here that Id4 expression is low in confluent preadipocytes and rapidly induced by treatment with the combination of hormones which causes differentiation into mature adipocytes. Id4 expression is also induced by treatment with individual hormones, especially dexamathasone. Id4 mRNA can be detected in mouse and human adipose tissue. Genes encoding E-proteins (bHLH proteins known to interact with and be regulated by Id proteins) are expressed and regulated during differentiation in 3T3-L1 cells. These data suggest that the Id4 transcriptional regulator is playing a role in adipose cell differentiation and suggest that DNA-binding HLH proteins may also be important in regulation of differentiation of these cells.",

author = "Heping Chen and Weng, {Yi Chinn} and Schatteman, {Gina C.} and Laura Sanders and Christy, {Robert J.} and Christy, {Barbara A.}",

note = "Funding Information: We thank Dr. Bihong Zhao for helpful discussions and suggestions. This work was supported in part by Research Grants R29HD29850 from the National Institutes of Health and 1-FY96-0126 from the March of Dimes Birth Defects Foundation (to B.A.C.) and by Grant JDF-K308299 from the Juvenile Diabetes Foundation (to R.J.C.) and a grant from the South Texas Health Research Center (to B.A.C. and R.J.C.).",

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AU - Chen, Heping

AU - Weng, Yi Chinn

AU - Schatteman, Gina C.

AU - Sanders, Laura

AU - Christy, Robert J.

AU - Christy, Barbara A.

N1 - Funding Information: We thank Dr. Bihong Zhao for helpful discussions and suggestions. This work was supported in part by Research Grants R29HD29850 from the National Institutes of Health and 1-FY96-0126 from the March of Dimes Birth Defects Foundation (to B.A.C.) and by Grant JDF-K308299 from the Juvenile Diabetes Foundation (to R.J.C.) and a grant from the South Texas Health Research Center (to B.A.C. and R.J.C.).

PY - 1999/3/24

Y1 - 1999/3/24

N2 - Expression of the Id4 gene was investigated during differentiation of 3T3-L1 preadipocytes into mature adipocytes. Id4 is a member of a family encoding non-DNA binding helix-loop-helix proteins proposed to inhibit the activity of basic HLH (bHLH) proteins important in many developmental processes. We show here that Id4 expression is low in confluent preadipocytes and rapidly induced by treatment with the combination of hormones which causes differentiation into mature adipocytes. Id4 expression is also induced by treatment with individual hormones, especially dexamathasone. Id4 mRNA can be detected in mouse and human adipose tissue. Genes encoding E-proteins (bHLH proteins known to interact with and be regulated by Id proteins) are expressed and regulated during differentiation in 3T3-L1 cells. These data suggest that the Id4 transcriptional regulator is playing a role in adipose cell differentiation and suggest that DNA-binding HLH proteins may also be important in regulation of differentiation of these cells.

AB - Expression of the Id4 gene was investigated during differentiation of 3T3-L1 preadipocytes into mature adipocytes. Id4 is a member of a family encoding non-DNA binding helix-loop-helix proteins proposed to inhibit the activity of basic HLH (bHLH) proteins important in many developmental processes. We show here that Id4 expression is low in confluent preadipocytes and rapidly induced by treatment with the combination of hormones which causes differentiation into mature adipocytes. Id4 expression is also induced by treatment with individual hormones, especially dexamathasone. Id4 mRNA can be detected in mouse and human adipose tissue. Genes encoding E-proteins (bHLH proteins known to interact with and be regulated by Id proteins) are expressed and regulated during differentiation in 3T3-L1 cells. These data suggest that the Id4 transcriptional regulator is playing a role in adipose cell differentiation and suggest that DNA-binding HLH proteins may also be important in regulation of differentiation of these cells.

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Expression of the dominant-negative regulator Id4 is induced during adipocyte differentiation

Abstract

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