Expression of the DNA repair gene XRCC1 in baboon tissues

Zi Qiang Zhou; Christi A. Walter

doi:10.1016/0165-7992(95)00053-4

Expression of the DNA repair gene XRCC1 in baboon tissues

Zi Qiang Zhou, Christi A. Walter

Department of Cell Systems & Anatomy

Research output: Contribution to journal › Article › peer-review

28 Scopus citations

Abstract

XRCC1 is a DNA repair gene involved in rejoining DNA strand-breaks. We used baboon as an animal model to determine the levels of XRCC1 gene expression in different tisues. Baboons were selected because they are evolutionarily closely related to humans. A single 2.2 kb transcript was detected in all tissues tested by northern blot analysis, with variations in levels of expression among tissues. The expression levels of XRCC1 were measured by quantitative RNase protection assays. XRCC1 mRNA levels were significantly higher in testis than those other tissues. A mean value of 24.6 × 10⁵ XRCC1 transcripts per μg DNA was found in testis, while 10.5 × 10⁵ in ovary, 9.8 × 10⁵ in brain, 8.5 × 10⁵ in liver, 6.8 × 10⁵ in kidney, 6.5 × 10⁵ in heart, 6.4 × 10⁵ in lymph nodes, 6.0 × 10⁵ in lung and 4.9 × 10⁵ in spleen were found.

Original language	English (US)
Pages (from-to)	111-116
Number of pages	6
Journal	Mutation Research Letters
Volume	348
Issue number	3
DOIs	https://doi.org/10.1016/0165-7992(95)00053-4
State	Published - Nov 1995

Keywords

Baboon testis expression
DNA repair gene expression
Quantitative RNAse protection assays
XRCC1

ASJC Scopus subject areas

Medicine(all)

Access to Document

10.1016/0165-7992(95)00053-4

Cite this

@article{736bbc2166f64f96b8d680f64ab414ae,

title = "Expression of the DNA repair gene XRCC1 in baboon tissues",

abstract = "XRCC1 is a DNA repair gene involved in rejoining DNA strand-breaks. We used baboon as an animal model to determine the levels of XRCC1 gene expression in different tisues. Baboons were selected because they are evolutionarily closely related to humans. A single 2.2 kb transcript was detected in all tissues tested by northern blot analysis, with variations in levels of expression among tissues. The expression levels of XRCC1 were measured by quantitative RNase protection assays. XRCC1 mRNA levels were significantly higher in testis than those other tissues. A mean value of 24.6 × 105 XRCC1 transcripts per μg DNA was found in testis, while 10.5 × 105 in ovary, 9.8 × 105 in brain, 8.5 × 105 in liver, 6.8 × 105 in kidney, 6.5 × 105 in heart, 6.4 × 105 in lymph nodes, 6.0 × 105 in lung and 4.9 × 105 in spleen were found.",

keywords = "Baboon testis expression, DNA repair gene expression, Quantitative RNAse protection assays, XRCC1",

author = "Zhou, {Zi Qiang} and Walter, {Christi A.}",

year = "1995",

month = nov,

doi = "10.1016/0165-7992(95)00053-4",

language = "English (US)",

volume = "348",

pages = "111--116",

journal = "Mutation Research Letters",

issn = "0165-7992",

publisher = "Elsevier BV",

number = "3",

}

TY - JOUR

T1 - Expression of the DNA repair gene XRCC1 in baboon tissues

AU - Zhou, Zi Qiang

AU - Walter, Christi A.

PY - 1995/11

Y1 - 1995/11

N2 - XRCC1 is a DNA repair gene involved in rejoining DNA strand-breaks. We used baboon as an animal model to determine the levels of XRCC1 gene expression in different tisues. Baboons were selected because they are evolutionarily closely related to humans. A single 2.2 kb transcript was detected in all tissues tested by northern blot analysis, with variations in levels of expression among tissues. The expression levels of XRCC1 were measured by quantitative RNase protection assays. XRCC1 mRNA levels were significantly higher in testis than those other tissues. A mean value of 24.6 × 105 XRCC1 transcripts per μg DNA was found in testis, while 10.5 × 105 in ovary, 9.8 × 105 in brain, 8.5 × 105 in liver, 6.8 × 105 in kidney, 6.5 × 105 in heart, 6.4 × 105 in lymph nodes, 6.0 × 105 in lung and 4.9 × 105 in spleen were found.

AB - XRCC1 is a DNA repair gene involved in rejoining DNA strand-breaks. We used baboon as an animal model to determine the levels of XRCC1 gene expression in different tisues. Baboons were selected because they are evolutionarily closely related to humans. A single 2.2 kb transcript was detected in all tissues tested by northern blot analysis, with variations in levels of expression among tissues. The expression levels of XRCC1 were measured by quantitative RNase protection assays. XRCC1 mRNA levels were significantly higher in testis than those other tissues. A mean value of 24.6 × 105 XRCC1 transcripts per μg DNA was found in testis, while 10.5 × 105 in ovary, 9.8 × 105 in brain, 8.5 × 105 in liver, 6.8 × 105 in kidney, 6.5 × 105 in heart, 6.4 × 105 in lymph nodes, 6.0 × 105 in lung and 4.9 × 105 in spleen were found.

KW - Baboon testis expression

KW - DNA repair gene expression

KW - Quantitative RNAse protection assays

KW - XRCC1

UR - http://www.scopus.com/inward/record.url?scp=0028838395&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0028838395&partnerID=8YFLogxK

U2 - 10.1016/0165-7992(95)00053-4

DO - 10.1016/0165-7992(95)00053-4

M3 - Article

C2 - 8524362

AN - SCOPUS:0028838395

VL - 348

SP - 111

EP - 116

JO - Mutation Research Letters

JF - Mutation Research Letters

SN - 0165-7992

IS - 3

ER -

Expression of the DNA repair gene XRCC1 in baboon tissues

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this