Expression of the DNA repair gene XRCC1 in baboon tissues

Zi Qiang Zhou, Christi A. Walter

Research output: Contribution to journalArticle

27 Scopus citations

Abstract

XRCC1 is a DNA repair gene involved in rejoining DNA strand-breaks. We used baboon as an animal model to determine the levels of XRCC1 gene expression in different tisues. Baboons were selected because they are evolutionarily closely related to humans. A single 2.2 kb transcript was detected in all tissues tested by northern blot analysis, with variations in levels of expression among tissues. The expression levels of XRCC1 were measured by quantitative RNase protection assays. XRCC1 mRNA levels were significantly higher in testis than those other tissues. A mean value of 24.6 × 105 XRCC1 transcripts per μg DNA was found in testis, while 10.5 × 105 in ovary, 9.8 × 105 in brain, 8.5 × 105 in liver, 6.8 × 105 in kidney, 6.5 × 105 in heart, 6.4 × 105 in lymph nodes, 6.0 × 105 in lung and 4.9 × 105 in spleen were found.

Original languageEnglish (US)
Pages (from-to)111-116
Number of pages6
JournalMutation Research Letters
Volume348
Issue number3
DOIs
StatePublished - Nov 1995

Keywords

  • Baboon testis expression
  • DNA repair gene expression
  • Quantitative RNAse protection assays
  • XRCC1

ASJC Scopus subject areas

  • Medicine(all)

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