Expression of the α2β1 and α3β1 integrins at the surface of mesothelial cells

A potential attachment site of endometrial cells

Craig A. Witz, Akiyuki Takahashi, Iris A. Montoya-Rodriguez, Sook Cho, Robert S Schenken

Research output: Contribution to journalArticle

40 Citations (Scopus)

Abstract

Objective: To localize α2β1 and α3β1 integrins in the cell membrane of peritoneal mesothelium in vivo and in vitro. Design: Descriptive study using confocal and two-photon laser-scanning microscopy. Setting: University- based laboratory. Patient(s): Women without endometriosis undergoing surgery for benign conditions. Intervention(s): None. Main Outcome Measure(s): Peritoneal biopsies (n = 9) and mesothelial monolayer cultures (n = 4) were incubated with antibodies to the α2 and α3 subunits and to the intact α2β1 and α3β1 integrins. Specimens were examined with laser-scanning microscopy. Result(s): The α2 and α3 subunits and the intact α2β1 and α3β1 integrins were identified at the base of the mesothelial cells (i.e., toward the basement membrane). There was also expression of the α2 and α3 subunits and the intact α2β1 and α3β1 integrins at the cell surface (i.e., toward the peritoneal cavity). Conclusion(s): The resolution of the confocal and two-photon laser-scanning microscope enabled localization of integrins in mesothelial cells. The presence of α2β1 (collagen-laminin receptor) and α3β1 integrins (collagen-laminin-fibronectin receptor) at the base of mesothelial cells suggests a role for these molecules in adhesion to the basement membrane. The presence of these molecules at the cell surface suggests a potential locus for cell adhesion in such processes as endometriosis and cancer metastasis. (C) 2000 by American Society for Reproductive Medicine.

Original languageEnglish (US)
Pages (from-to)579-584
Number of pages6
JournalFertility and Sterility
Volume74
Issue number3
DOIs
StatePublished - Sep 2000

Fingerprint

Integrins
Endometriosis
Photons
Basement Membrane
Confocal Microscopy
Collagen Receptors
Integrin alpha5beta1
Laminin Receptors
Peritoneal Cavity
Laminin
Cell Adhesion
Lasers
Collagen
Epithelium
Cell Membrane
Outcome Assessment (Health Care)
Neoplasm Metastasis
Biopsy
Antibodies
Neoplasms

Keywords

  • Cancer metastasis
  • Confocal
  • Endometriosis
  • Integrin
  • Laser-scanning microscopy
  • Mesothelium
  • Peritoneum

ASJC Scopus subject areas

  • Obstetrics and Gynecology

Cite this

Expression of the α2β1 and α3β1 integrins at the surface of mesothelial cells : A potential attachment site of endometrial cells. / Witz, Craig A.; Takahashi, Akiyuki; Montoya-Rodriguez, Iris A.; Cho, Sook; Schenken, Robert S.

In: Fertility and Sterility, Vol. 74, No. 3, 09.2000, p. 579-584.

Research output: Contribution to journalArticle

Witz, Craig A. ; Takahashi, Akiyuki ; Montoya-Rodriguez, Iris A. ; Cho, Sook ; Schenken, Robert S. / Expression of the α2β1 and α3β1 integrins at the surface of mesothelial cells : A potential attachment site of endometrial cells. In: Fertility and Sterility. 2000 ; Vol. 74, No. 3. pp. 579-584.
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