Expression of simian virus 40 T antigen in insect cells using a baculovirus expression vector

Robert E. Lanford

Research output: Contribution to journalArticle

101 Citations (Scopus)

Abstract

Simian virus 40 (SV40) large T and small t antigens were synthesized in insect cells using the baculovirus Autographa californica as an expression vector. A recombinant virus containing a genomic copy of the SV40 early region expressed high levels of small t antigen but only low levels of large T antigen. However, very high levels of T antigen synthesis were observed when viruses were constructed with a cDNA copy of the large T antigen mRNA. Insect cells were capable of modifying T antigen by phosphorylation, palmitylation, glycosylation, and oligomerization. Functional assays demonstrated that the origin-specific DNA binding, ATPase, and helicase activities of insect cell-derived T antigen were comparable to T antigen synthesized in mammalian cells. Use of the baculovirus vector system to produce T antigen should facilitate future investigations requiring large quantities of T antigen.

Original languageEnglish (US)
Pages (from-to)72-81
Number of pages10
JournalVirology
Volume167
Issue number1
DOIs
StatePublished - 1988
Externally publishedYes

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Simian virus 40
Baculoviridae
Viral Tumor Antigens
Insects
Viruses
Glycosylation
Adenosine Triphosphatases
Complementary DNA
Phosphorylation
Antigens
Messenger RNA
DNA

ASJC Scopus subject areas

  • Virology
  • Infectious Diseases

Cite this

Expression of simian virus 40 T antigen in insect cells using a baculovirus expression vector. / Lanford, Robert E.

In: Virology, Vol. 167, No. 1, 1988, p. 72-81.

Research output: Contribution to journalArticle

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