Expression of Scl in mesoderm rescues hematopoiesis in the absence of Oct-4

Kimi Y. Kong, Elizabeth A. Williamson, Jason H. Rogers, Tam Tran, Robert A Hromas, Richard Dahl

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

In embryonic stem cells, Oct-4 concentration is critical in determining the development of endoderm, mesoderm, and trophectoderm. Although Oct-4 expression is essential for mesoderm development, it is unclear whether it has a role in the development of specific mesodermal tissues. In this study, we have examined the importance of Oct-4 in the generation of hematopoietic cells using an inducible Oct-4 ESC line.We demonstrate that Oct-4 has a role in supporting hematopoiesis after specifying brachyury-positive mesoderm. When we suppressed Oct-4 expression before or after mesoderm specification, no hematopoietic cells are detected. However, hematopoiesis can be rescued in the absence of Oct-4 after mesoderm specification if the essential hematopoietic transcription factor stem cell leukemia is expressed. Our results suggest that, for hematopoiesis to occur, Oct-4 is required for the initial specification of mesoderm and subsequently is required for the development of hematopoietic cells from uncommitted mesoderm.

Original languageEnglish (US)
Pages (from-to)60-63
Number of pages4
JournalBlood
Volume114
Issue number1
DOIs
StatePublished - Nov 18 2009
Externally publishedYes

Fingerprint

Hematopoiesis
Mesoderm
Stem cells
Specifications
Transcription Factors
Tissue
Endoderm
Embryonic Stem Cells
Leukemia
Stem Cells

ASJC Scopus subject areas

  • Biochemistry
  • Immunology
  • Hematology
  • Cell Biology

Cite this

Kong, K. Y., Williamson, E. A., Rogers, J. H., Tran, T., Hromas, R. A., & Dahl, R. (2009). Expression of Scl in mesoderm rescues hematopoiesis in the absence of Oct-4. Blood, 114(1), 60-63. https://doi.org/10.1182/blood-2008-08-174755

Expression of Scl in mesoderm rescues hematopoiesis in the absence of Oct-4. / Kong, Kimi Y.; Williamson, Elizabeth A.; Rogers, Jason H.; Tran, Tam; Hromas, Robert A; Dahl, Richard.

In: Blood, Vol. 114, No. 1, 18.11.2009, p. 60-63.

Research output: Contribution to journalArticle

Kong, KY, Williamson, EA, Rogers, JH, Tran, T, Hromas, RA & Dahl, R 2009, 'Expression of Scl in mesoderm rescues hematopoiesis in the absence of Oct-4', Blood, vol. 114, no. 1, pp. 60-63. https://doi.org/10.1182/blood-2008-08-174755
Kong, Kimi Y. ; Williamson, Elizabeth A. ; Rogers, Jason H. ; Tran, Tam ; Hromas, Robert A ; Dahl, Richard. / Expression of Scl in mesoderm rescues hematopoiesis in the absence of Oct-4. In: Blood. 2009 ; Vol. 114, No. 1. pp. 60-63.
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