Expression of Scl in mesoderm rescues hematopoiesis in the absence of Oct-4

Kimi Y. Kong, Elizabeth A. Williamson, Jason H. Rogers, Tam Tran, Robert Hromas, Richard Dahl

Research output: Contribution to journalArticle

6 Scopus citations

Abstract

In embryonic stem cells, Oct-4 concentration is critical in determining the development of endoderm, mesoderm, and trophectoderm. Although Oct-4 expression is essential for mesoderm development, it is unclear whether it has a role in the development of specific mesodermal tissues. In this study, we have examined the importance of Oct-4 in the generation of hematopoietic cells using an inducible Oct-4 ESC line.We demonstrate that Oct-4 has a role in supporting hematopoiesis after specifying brachyury-positive mesoderm. When we suppressed Oct-4 expression before or after mesoderm specification, no hematopoietic cells are detected. However, hematopoiesis can be rescued in the absence of Oct-4 after mesoderm specification if the essential hematopoietic transcription factor stem cell leukemia is expressed. Our results suggest that, for hematopoiesis to occur, Oct-4 is required for the initial specification of mesoderm and subsequently is required for the development of hematopoietic cells from uncommitted mesoderm.

Original languageEnglish (US)
Pages (from-to)60-63
Number of pages4
JournalBlood
Volume114
Issue number1
DOIs
StatePublished - Nov 18 2009

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ASJC Scopus subject areas

  • Biochemistry
  • Immunology
  • Hematology
  • Cell Biology

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