Expression of platelet-derived growth factor-B mRNA during vaginal vs. dermal incisional wound healing in the rabbit

Yoram Abramov, Emmet Hirsch, Vladimir Ilievski, Roger P. Goldberg, Sylvia M. Botros, Peter K. Sand

Research output: Contribution to journalArticle

8 Scopus citations

Abstract

Objective: The outcome of pelvic reconstructive surgery is largely dependent on the vaginal wound healing process, but this process has not yet been fully elucidated. Platelet-derived growth factor (PDGF) is an important mediator of the wound healing process in cutaneous tissue. We sought to compare PDGF-B mRNA expression in vaginal versus cutaneous incisional wound healing in a rabbit model. Study design: Bilateral 6 mm full-thickness circular segments were excised from the vagina and abdominal skin in 36 New Zealand-White female rabbits. Animals were euthanized sequentially before, on the day of and 4, 7, 10, 14, 21, 28 and 35 days after wounding. Their wounds were evaluated for surface area and PDGF-B mRNA expression using real time PCR. Results: In both tissues PDGF-B mRNA expression increased constantly after wounding, reaching peak levels on day 10, and declined immediately thereafter, reaching minimal values on day 21. In both tissues, the expression of PDGF-B mRNA significantly correlated with the wound closure rate. Conclusion: PDGF-B mRNA expression significantly correlates with incisional vaginal and cutaneous wound closure, suggesting that this factor plays an important role in the wound healing process of both tissues.

Original languageEnglish (US)
Pages (from-to)216-220
Number of pages5
JournalEuropean Journal of Obstetrics and Gynecology and Reproductive Biology
Volume162
Issue number2
DOIs
StatePublished - Jun 2012
Externally publishedYes

Keywords

  • Abdomen
  • Platelet derived growth factor
  • Rabbit
  • Skin
  • Vagina
  • Wound healing

ASJC Scopus subject areas

  • Reproductive Medicine
  • Obstetrics and Gynecology

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