Expression of NADPH oxidase isoform 1 (Nox1) in human placenta: Involvement in preeclampsia

X. L. Cui, D. Brockman, B. Campos, L. Myatt

Research output: Contribution to journalArticle

62 Citations (Scopus)

Abstract

Increased oxidative stress in the placenta has been associated with preeclampsia (PE), a clinical syndrome involving placental pathology. The enzymatic sources of reactive oxygen species in the human placenta are as yet unidentified. We hypothesized that NADPH oxidase is a main source of reactive oxygen species in the placenta and its expression may change in PE. Employing RT-PCR, we have amplified a novel NADPH oxidase isoform Nox1 from human choriocarcinoma BeWo cells. Using polyclonal anti-peptide antiserum recognizing unique Nox1 peptide sequences, we identified by immunohistochemistry and cell fractionation that Nox1 protein localizes in the BeWo cell membrane structures. Immunohistochemistry of normal placental tissues showed that Nox1 was localized in syncytiotrophoblasts, in villous vascular endothelium, and in some stromal cells. At the immunohistochemical level Nox1 expression was significantly increased in syncytiotrophoblast and endothelial cells in placentas from patients with preeclampsia as compared to gestational age-matched controls. Western blot analysis of whole placental homogenate confirmed this increase. Our data suggests that increased Nox1 expression is associated with the increased oxidative stress found in these placentas.

Original languageEnglish (US)
Pages (from-to)422-431
Number of pages10
JournalPlacenta
Volume27
Issue number4-5
DOIs
StatePublished - Apr 2006
Externally publishedYes

Fingerprint

Pre-Eclampsia
Placenta
Protein Isoforms
NADPH Oxidase
Trophoblasts
Reactive Oxygen Species
Oxidative Stress
Immunohistochemistry
Cell Membrane Structures
Cell Fractionation
Peptides
Choriocarcinoma
Vascular Endothelium
Stromal Cells
NADPH oxidase 1
Gestational Age
Immune Sera
Endothelial Cells
Western Blotting
Pathology

Keywords

  • NADPH oxidase
  • Oxidative stress
  • Placenta
  • Preeclampsia
  • Trophoblast

ASJC Scopus subject areas

  • Obstetrics and Gynecology

Cite this

Expression of NADPH oxidase isoform 1 (Nox1) in human placenta : Involvement in preeclampsia. / Cui, X. L.; Brockman, D.; Campos, B.; Myatt, L.

In: Placenta, Vol. 27, No. 4-5, 04.2006, p. 422-431.

Research output: Contribution to journalArticle

Cui, X. L. ; Brockman, D. ; Campos, B. ; Myatt, L. / Expression of NADPH oxidase isoform 1 (Nox1) in human placenta : Involvement in preeclampsia. In: Placenta. 2006 ; Vol. 27, No. 4-5. pp. 422-431.
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