Expression of monocyte chemoattractant protein 1 in human osteoblastic cells stimulated by proinflammatory mediators

Jian‐Fu ‐F Zhu, Anthony J. Valente, Joseph A. Lorenzo, David Carnes, Dana T. Graves

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60 Scopus citations


Monocyte chemoattractant protein 1 (MCP‐1) is a member of the chemokine superfamily of genes that induces chemotaxis of monocytes in inflammatory processes. The effects of interleukin‐1β (IL‐1β), tumor necrosis factor α (TNF‐α), interleukin‐6 (IL‐6), transforming growth factor β (TGF‐β), platelet‐derived growth factor (PDGF‐BB), parathyroid hormone (PTH), and 1,25(OH)2D3 on MCP‐1 expression in human osteoblastic cells were compared. Inflammatory or proinflammatory cytokines stimulated the production of MCP‐1 in normal human osteoblastic cells as determined by RIA. The osteotrophic mediators PTH and 1,25(OH)2D3 and PDGF‐BB had no effect on MCP‐1 expression. In further studies, the steady‐state mRNA and MCP‐1 protein levels in two human osteoblastic cell lines, MG‐63 and SaOS‐2, were examined. MCP‐1 expression at both the protein and mRNA levels was greatly increased by IL‐1β and TNF‐α. At the mRNA level, IL‐1β and TNF‐α strongly induced MCP‐1 expression; TGF‐β and IL‐6 induced MCP‐1 but to a lesser extent. No significant changes in MCP‐1 mRNA or MCP‐1 protein secretion were observed when cells were treated with PDGF‐BB, PTH, and 1,25(OH)2D3. When tested on preosteoclasts, MCP‐1 was shown to have no effect on the formation of multinucleated, tartrate‐resistant acid phosphatase (TRAP)‐positive osteoclastic cells.

Original languageEnglish (US)
Pages (from-to)1123-1130
Number of pages8
JournalJournal of Bone and Mineral Research
Issue number7
StatePublished - Jul 1994
Externally publishedYes

ASJC Scopus subject areas

  • Endocrinology, Diabetes and Metabolism
  • Orthopedics and Sports Medicine


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