Expression of latent hematopoietic progenitor cells in cultures of newborn and adult baboon liver

G. D. Roodman, J. L. VandeBerg, T. J. Kuehl

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

The anatomic site of hematopoiesis changes during fetal development from the yolk sac to the liver and finally to the marrow. Factors controlling this switch in the site of hematopoiesis are unknown. We assayed erythroid colony (CFU-E) and erythroid burst (BFU-E) formation in fetal, newborn, and adult baboon liver and marrow to determine the growth requirements of primate hematopoietic progenitor cells from different anatomic sites and developmental stages. We cocultured fetal, newborn, and adult liver and marrow nonadherent cells with adherent cells from these organs to assess the role adherent cells may play in determining the site of hematopoiesis. Fetal liver, fetal marrow, newborn marrow, and adult marrow cultures formed CFU-E and BFU-E colonies in vitro. In contrast, newborn and adult liver cell cultures very rarely formed colonies. However, when newborn adult liver nonadherent cells were cocultured with marrow adherent cells, CFU-E and BFU-E colonies were detected. The colonies that formed in the newborn and adult liver cultures were derived from the liver and not from the marrow cells or peripheral blood trapped in the liver. These data suggest that in contrast to fetal liver, newborn and adult liver may not be hematopoietic organs in normal primates in vivo because of changes in the growth requirements of hematopoietic progenitor cells present in these organs.

Original languageEnglish (US)
Pages (from-to)1518-1525
Number of pages8
JournalBlood
Volume65
Issue number6
StatePublished - 1985
Externally publishedYes

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Papio
Hematopoietic Stem Cells
Liver
Cell Culture Techniques
Erythroid Precursor Cells
Bone Marrow
Hematopoiesis
Primates
Yolk Sac
Growth
Fetal Development
Cell culture
Blood
Cells
Switches

ASJC Scopus subject areas

  • Hematology

Cite this

Roodman, G. D., VandeBerg, J. L., & Kuehl, T. J. (1985). Expression of latent hematopoietic progenitor cells in cultures of newborn and adult baboon liver. Blood, 65(6), 1518-1525.

Expression of latent hematopoietic progenitor cells in cultures of newborn and adult baboon liver. / Roodman, G. D.; VandeBerg, J. L.; Kuehl, T. J.

In: Blood, Vol. 65, No. 6, 1985, p. 1518-1525.

Research output: Contribution to journalArticle

Roodman, GD, VandeBerg, JL & Kuehl, TJ 1985, 'Expression of latent hematopoietic progenitor cells in cultures of newborn and adult baboon liver', Blood, vol. 65, no. 6, pp. 1518-1525.
Roodman, G. D. ; VandeBerg, J. L. ; Kuehl, T. J. / Expression of latent hematopoietic progenitor cells in cultures of newborn and adult baboon liver. In: Blood. 1985 ; Vol. 65, No. 6. pp. 1518-1525.
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