Expression of interleukin-10 in human gestational tissues

Michael S. Trautman, Debbie Collmer, Sam S. Edwin, William White, Murray D. Mitchell, Donald J. Dudley

Research output: Contribution to journalArticlepeer-review

62 Scopus citations

Abstract

OBJECTIVE: To determine production of interleukin-10 (IL-10) by interleukin-1β (IL-1β) in cultured decidual, chorion, and amnion cells and whether IL-10 is produced in gestational tissues under the setting of infection-associated preterm labor. METHODS: Decidual, chorion, and amnion cells were isolated from term placentas and grown in primary culture. The cells were incubated with various concentrations of IL-1β and then culture supernatants were assayed for IL-10 by enzyme-linked immunosorbent assay. In subsequent studies, gestational membranes were isolated from a normal-term pregnancy and a preterm pregnancy complicated by chorioamnionitis. Tissues were evaluated for IL-10 expression by immunohistology and in situ hybridization. Human gestational tissues were collected from 38 women experiencing: 1) term cesarean delivery without labor; 2) normal-term vaginal delivery; 3) preterm cesarean delivery without labor; 4) preterm vaginal delivery without chorioamnionitis; and 5) preterm vaginal delivery with concomitant chorioamnionitis. Amnion, chorion, and decidua were isolated, total RNA from each tissue was extracted, and the presence of IL-10 mRNA was determined by reverse transcriptase-polymerase chain reaction (RT-PCR). RESULTS: Decidual cells in culture produced IL-10 in response to IL-1β, but chorion and amnion cells produced no IL-10 protein. In vivo protein expression by immunohistology showed that most protein was detected within decidua while cells within amnion and chorion rarely had detectable IL-10 protein. In vivo RT-PCR samples demonstrated the strongest IL-10 mRNA signal from decidua samples, although IL-10 mRNA was also noted in chorion and amnion of placentas obtained after preterm labor. CONCLUSION: Maternal decidual cells can potentially produce IL-10, but fetal membranes (amnion and chorion) appear to have limited capabilities to produce IL-10. The relative inability of fetal tissues to produce IL-10 may play an important role in the pathophysiology of infection-associated preterm labor.

Original languageEnglish (US)
Pages (from-to)247-253
Number of pages7
JournalJournal of the Society for Gynecologic Investigation
Volume4
Issue number5
DOIs
StatePublished - Sep 1997
Externally publishedYes

Keywords

  • Chorion
  • Cytokines
  • Decidua
  • Interleukin-10
  • Preterm labor

ASJC Scopus subject areas

  • Obstetrics and Gynecology

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