Expression of infectious viral particles by primary chimpanzee hepatocytes isolated during the acute phase of non-A, non-B hepatitis

James R. Jacob, Kenneth H. Burk, Jorg W. Eichberg, Gordon R. Dreesman, Robert E. Lanford

Research output: Contribution to journalArticle

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Abstract

Liver wedge biopsies were obtained from chimpanzees during the acute phase of experimental non-A, non-B hepatitis infections. Primary chimpanzee hepatocytes were maintained for over 4 weeks in vitro with a serum-free medium supplemented with growth factors and hormones. The de novo synthesis and secretion of plasma proteins characteristic for differentiated primate hepatocytes were sustained under these culture conditions. Immunocytochemical staining for a non-A, non-B hepatitis-associated antigen revealed expression of this cytoplasmic marker during the culture period, indicating a persistence of the infection in vitro. Tissue culture medium derived from the hepatocyte cultures was used to inoculate a nonimmune chimpanzee. The animal subsequently displayed an increase in the serum levels of alanine aminotransferase, the development of histopathologic alterations indicative of viral hepatitis, and the appearance of liver cell cytoplasmic tubules diagnostic for non-A, non-B hepatitis. Concentrated tissue culture medium examined by electron microscopy contained virus-like particles with an average diameter of 39-46 nm, which exhibited an envelope and inner 37-nm core structure.

Original languageEnglish (US)
Pages (from-to)1121-1127
Number of pages7
JournalJournal of Infectious Diseases
Volume161
Issue number6
StatePublished - Jun 1990
Externally publishedYes

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Pan troglodytes
Virion
Hepatitis
Hepatocytes
Culture Media
Hepatitis C Antigens
Liver
Serum-Free Culture Media
Infection
Alanine Transaminase
Primates
Growth Hormone
Blood Proteins
Intercellular Signaling Peptides and Proteins
Electron Microscopy
Staining and Labeling
Biopsy
Serum
In Vitro Techniques

ASJC Scopus subject areas

  • Public Health, Environmental and Occupational Health
  • Immunology

Cite this

Jacob, J. R., Burk, K. H., Eichberg, J. W., Dreesman, G. R., & Lanford, R. E. (1990). Expression of infectious viral particles by primary chimpanzee hepatocytes isolated during the acute phase of non-A, non-B hepatitis. Journal of Infectious Diseases, 161(6), 1121-1127.

Expression of infectious viral particles by primary chimpanzee hepatocytes isolated during the acute phase of non-A, non-B hepatitis. / Jacob, James R.; Burk, Kenneth H.; Eichberg, Jorg W.; Dreesman, Gordon R.; Lanford, Robert E.

In: Journal of Infectious Diseases, Vol. 161, No. 6, 06.1990, p. 1121-1127.

Research output: Contribution to journalArticle

Jacob, James R. ; Burk, Kenneth H. ; Eichberg, Jorg W. ; Dreesman, Gordon R. ; Lanford, Robert E. / Expression of infectious viral particles by primary chimpanzee hepatocytes isolated during the acute phase of non-A, non-B hepatitis. In: Journal of Infectious Diseases. 1990 ; Vol. 161, No. 6. pp. 1121-1127.
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