Expression of IL-18 by SIV does not modify the outcome of the antiviral immune response

Luis D. Giavedoni, M. Cristina Velasquillo, Laura M. Parodi, Gene B. Hubbard, Vida L. Hodara

    Research output: Contribution to journalArticle

    9 Scopus citations

    Abstract

    Interleukin 18 (IL-18) is a proinflammatory cytokine expressed by several cell types, including activated dendritic cells and macrophages, that acts in synergy with IL-12 as an important amplifying factor for IFN-γ production and Th1 development. To study the immunological and virological effects of IL-18 expression in the context of a lentiviral infection, we inoculated rhesus macaques with a high dose of replication-competent simian immunodeficiency virus (SIV) vectors carrying the rhesus IL-18 gene in the sense (SIVIL-18) or antisense (SIVFIGI) orientation. Both vectors behaved as attenuated viruses, resulting in low viral loads, induction of low and transient levels of inflammatory cytokines, no CD4+ T cell depletion, and mild activation of T lymphocytes. Although IL-18-expressing virus could be isolated from some SIVIL18-infected macaques for 12 weeks postinfection, the anti-SIV humoral and cellular immune responses of macaques inoculated with SIVIL18 and SIVFIGI were similar to each other, with the exception of an early IFN-γ response in animals infected with SIVIL18. In summary, expression of IL-18 during the acute phase of SIV infection does not increase viral replication or influence the outcome of the antiviral immune response.

    Original languageEnglish (US)
    Pages (from-to)327-337
    Number of pages11
    JournalVirology
    Volume303
    Issue number2
    DOIs
    StatePublished - Jan 1 2002

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    Keywords

    • Antibodies
    • CTL
    • Cytokines
    • IL-18
    • Immune response
    • Live-attenuated virus
    • Proliferation
    • Rhesus macaques
    • SIV
    • Vaccine

    ASJC Scopus subject areas

    • Virology

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