Abstract
Interleukin 18 (IL-18) is a proinflammatory cytokine expressed by several cell types, including activated dendritic cells and macrophages, that acts in synergy with IL-12 as an important amplifying factor for IFN-γ production and Th1 development. To study the immunological and virological effects of IL-18 expression in the context of a lentiviral infection, we inoculated rhesus macaques with a high dose of replication-competent simian immunodeficiency virus (SIV) vectors carrying the rhesus IL-18 gene in the sense (SIVIL-18) or antisense (SIVFIGI) orientation. Both vectors behaved as attenuated viruses, resulting in low viral loads, induction of low and transient levels of inflammatory cytokines, no CD4+ T cell depletion, and mild activation of T lymphocytes. Although IL-18-expressing virus could be isolated from some SIVIL18-infected macaques for 12 weeks postinfection, the anti-SIV humoral and cellular immune responses of macaques inoculated with SIVIL18 and SIVFIGI were similar to each other, with the exception of an early IFN-γ response in animals infected with SIVIL18. In summary, expression of IL-18 during the acute phase of SIV infection does not increase viral replication or influence the outcome of the antiviral immune response.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 327-337 |
| Number of pages | 11 |
| Journal | Virology |
| Volume | 303 |
| Issue number | 2 |
| DOIs | |
| State | Published - 2002 |
| Externally published | Yes |
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Keywords
- Antibodies
- CTL
- Cytokines
- IL-18
- Immune response
- Live-attenuated virus
- Proliferation
- Rhesus macaques
- SIV
- Vaccine
ASJC Scopus subject areas
- Virology
- Infectious Diseases
Cite this
Expression of IL-18 by SIV does not modify the outcome of the antiviral immune response. / Giavedoni, Luis D.; Velasquillo, M. Cristina; Parodi, Laura M.; Hubbard, Gene B.; Hodara, Vida L.
In: Virology, Vol. 303, No. 2, 2002, p. 327-337.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Expression of IL-18 by SIV does not modify the outcome of the antiviral immune response
AU - Giavedoni, Luis D.
AU - Velasquillo, M. Cristina
AU - Parodi, Laura M.
AU - Hubbard, Gene B.
AU - Hodara, Vida L.
PY - 2002
Y1 - 2002
N2 - Interleukin 18 (IL-18) is a proinflammatory cytokine expressed by several cell types, including activated dendritic cells and macrophages, that acts in synergy with IL-12 as an important amplifying factor for IFN-γ production and Th1 development. To study the immunological and virological effects of IL-18 expression in the context of a lentiviral infection, we inoculated rhesus macaques with a high dose of replication-competent simian immunodeficiency virus (SIV) vectors carrying the rhesus IL-18 gene in the sense (SIVIL-18) or antisense (SIVFIGI) orientation. Both vectors behaved as attenuated viruses, resulting in low viral loads, induction of low and transient levels of inflammatory cytokines, no CD4+ T cell depletion, and mild activation of T lymphocytes. Although IL-18-expressing virus could be isolated from some SIVIL18-infected macaques for 12 weeks postinfection, the anti-SIV humoral and cellular immune responses of macaques inoculated with SIVIL18 and SIVFIGI were similar to each other, with the exception of an early IFN-γ response in animals infected with SIVIL18. In summary, expression of IL-18 during the acute phase of SIV infection does not increase viral replication or influence the outcome of the antiviral immune response.
AB - Interleukin 18 (IL-18) is a proinflammatory cytokine expressed by several cell types, including activated dendritic cells and macrophages, that acts in synergy with IL-12 as an important amplifying factor for IFN-γ production and Th1 development. To study the immunological and virological effects of IL-18 expression in the context of a lentiviral infection, we inoculated rhesus macaques with a high dose of replication-competent simian immunodeficiency virus (SIV) vectors carrying the rhesus IL-18 gene in the sense (SIVIL-18) or antisense (SIVFIGI) orientation. Both vectors behaved as attenuated viruses, resulting in low viral loads, induction of low and transient levels of inflammatory cytokines, no CD4+ T cell depletion, and mild activation of T lymphocytes. Although IL-18-expressing virus could be isolated from some SIVIL18-infected macaques for 12 weeks postinfection, the anti-SIV humoral and cellular immune responses of macaques inoculated with SIVIL18 and SIVFIGI were similar to each other, with the exception of an early IFN-γ response in animals infected with SIVIL18. In summary, expression of IL-18 during the acute phase of SIV infection does not increase viral replication or influence the outcome of the antiviral immune response.
KW - Antibodies
KW - CTL
KW - Cytokines
KW - IL-18
KW - Immune response
KW - Live-attenuated virus
KW - Proliferation
KW - Rhesus macaques
KW - SIV
KW - Vaccine
UR - http://www.scopus.com/inward/record.url?scp=0036941372&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0036941372&partnerID=8YFLogxK
U2 - 10.1006/viro.2002.1647
DO - 10.1006/viro.2002.1647
M3 - Article
VL - 303
SP - 327
EP - 337
JO - Virology
JF - Virology
SN - 0042-6822
IS - 2
ER -