Expression of heat shock protein 70 decreases with age in hepatocytes and splenocytes from female rats

Astrid Gutsmann-Conrad, Mohammad A. Pahlavani, Ahmad R. Heydari, Arlan Richardson

Research output: Contribution to journalArticle

29 Scopus citations

Abstract

A decline in the induction of heat shock protein 70 (hsp70) expression with age has been shown to occur in a variety of tissues from male rodents. Because the age-related change in the expression of many genes often differ in male and female rodents, we have measured the induction of hsp70 expression in hepatocytes and splenocytes from young/adult (4-8 months) and old (20-22 months) female Fischer 344 rats. Hepatocytes and splenocytes isolated from old female rats showed a marked decrease in the induction of hsp70 mRNA and protein levels by heat shock when compared to hepatocytes and splenocytes isolated from young/adult female rats. Because the heat shock transcription factor HSF1 mediates the heat-induced transcription of hsp70, the effect of age on HSF1 was also studied. The ability of extracts from heat-shocked splenocytes to bind to the heat shock element (HSE) decreased with age. Interestingly, the levels of HSF1 protein were similar in splenocytes and hepatocytes from old female rats compared to young/adult female rats, even though the levels of HSE-binding were lower for splenocytes isolated from old rats. In this study, we show an age-related decline in the expression of hsp70, and this decline was similar to what we had previously observed in male Fischer 344 rats.

Original languageEnglish (US)
Pages (from-to)255-270
Number of pages16
JournalMechanisms of Ageing and Development
Volume107
Issue number3
DOIs
StatePublished - Mar 15 1999

Keywords

  • Heat shock proteins
  • Heat shock transcription factor
  • Hsp70
  • Liver
  • Spleen

ASJC Scopus subject areas

  • Aging
  • Developmental Biology

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