Expression of glucocorticoid and progesterone nuclear receptor genes in archival breast cancer tissue

Robert A. Smith, Rod A. Lea, Joanne E. Curran, Stephen R. Weinstein, Lyn R. Griffiths

Research output: Contribution to journalArticle

15 Scopus citations

Abstract

Background: Previous studies in our laboratory have shown associations of specific nuclear receptor gene variants with sporadic breast cancer. In order to investigate these findings further, we conducted the present study to determine whether expression levels of the progesterone and glucocorticoid nuclear receptor genes vary in different breast cancer grades. Methods: RNA was extracted from paraffin-embedded archival breast tumour tissue and converted into cDNA. Sample cDNA underwent PCR using labelled primers to enable quantitation of mRNA expression. Expression data were normalized against the 18S ribosomal gene multiplex and analyzed using analysis of variance. Results: Analysis of variance indicated a variable level of expression of both genes with regard to breast cancer grade (P = 0.00033 for glucocorticoid receptor and P = 0.023 for progesterone receptor). Conclusion: Statistical analysis indicated that expression of the progesterone nuclear receptor is elevated in late grade breast cancer tissue.

Original languageEnglish (US)
Article numberR9
JournalBreast Cancer Research
Volume5
Issue number1
DOIs
StatePublished - Dec 4 2002
Externally publishedYes

Keywords

  • Breast cancer
  • Glucocorticoid receptor
  • Nuclear receptors
  • Progesterone receptor

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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    Smith, R. A., Lea, R. A., Curran, J. E., Weinstein, S. R., & Griffiths, L. R. (2002). Expression of glucocorticoid and progesterone nuclear receptor genes in archival breast cancer tissue. Breast Cancer Research, 5(1), [R9]. https://doi.org/10.1186/bcr556