Expression of glucocorticoid and progesterone nuclear receptor genes in archival breast cancer tissue.

Robert A. Smith, Rod A. Lea, Joanne E. Curran, Stephen R. Weinstein, Lyn R. Griffiths

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

BACKGROUND: Previous studies in our laboratory have shown associations of specific nuclear receptor gene variants with sporadic breast cancer. In order to investigate these findings further, we conducted the present study to determine whether expression levels of the progesterone and glucocorticoid nuclear receptor genes vary in different breast cancer grades. METHODS: RNA was extracted from paraffin-embedded archival breast tumour tissue and converted into cDNA. Sample cDNA underwent PCR using labelled primers to enable quantitation of mRNA expression. Expression data were normalized against the 18S ribosomal gene multiplex and analyzed using analysis of variance. RESULTS: Analysis of variance indicated a variable level of expression of both genes with regard to breast cancer grade (P = 0.00033 for glucocorticoid receptor and P = 0.023 for progesterone receptor). CONCLUSION: Statistical analysis indicated that expression of the progesterone nuclear receptor is elevated in late grade breast cancer tissue.

Original languageEnglish (US)
JournalBreast cancer research : BCR
Volume5
Issue number1
StatePublished - 2003
Externally publishedYes

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Progesterone Receptors
Cytoplasmic and Nuclear Receptors
Glucocorticoids
Breast Neoplasms
Genes
Glucocorticoid Receptors
Analysis of Variance
Complementary DNA
Paraffin
Progesterone
RNA
Gene Expression
Polymerase Chain Reaction
Messenger RNA

Cite this

Smith, R. A., Lea, R. A., Curran, J. E., Weinstein, S. R., & Griffiths, L. R. (2003). Expression of glucocorticoid and progesterone nuclear receptor genes in archival breast cancer tissue. Breast cancer research : BCR, 5(1).

Expression of glucocorticoid and progesterone nuclear receptor genes in archival breast cancer tissue. / Smith, Robert A.; Lea, Rod A.; Curran, Joanne E.; Weinstein, Stephen R.; Griffiths, Lyn R.

In: Breast cancer research : BCR, Vol. 5, No. 1, 2003.

Research output: Contribution to journalArticle

Smith, RA, Lea, RA, Curran, JE, Weinstein, SR & Griffiths, LR 2003, 'Expression of glucocorticoid and progesterone nuclear receptor genes in archival breast cancer tissue.', Breast cancer research : BCR, vol. 5, no. 1.
Smith, Robert A. ; Lea, Rod A. ; Curran, Joanne E. ; Weinstein, Stephen R. ; Griffiths, Lyn R. / Expression of glucocorticoid and progesterone nuclear receptor genes in archival breast cancer tissue. In: Breast cancer research : BCR. 2003 ; Vol. 5, No. 1.
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