Expression of dopamine D(1A) receptor gene in proximal tubule of rat kidneys

I. Yamaguchi, P. A. Jose, M. M. Mouradian, L. M. Canessa, F. J. Monsma, D. R. Sibley, K. Takeyasu, R. A. Felder

Research output: Contribution to journalArticle

56 Scopus citations

Abstract

The existence of dopamine receptor subtypes outside the central nervous system has been suggested by pharmacological and biochemical techniques. Whether the renal dopamine receptors are distinct from those cloned from the brain remains to be determined. Indeed, the expression of any of these receptor genes in the kidney has not been demonstrated definitively. In this study, we amplified D(1A) receptor cDNA from microdissected proximal convoluted tubules of the rat kidney by reverse transcription-polymerase chain reaction; primers were based on the sequence of rat D(1A) cDNA cloned from the brain and corresponded to the third cytoplasmic loop of the receptor. Specificity of the amplified products was verified by restriction analysis, Southern blots, and sequencing. Furthermore, solution hybridization indicated the presence of a single ribonuclease-protected RNA species corresponding to the D(1A) receptor mRNA in proximal tubules. These studies report for the first time the presence of D(1A) receptor message in the kidney.

Original languageEnglish (US)
Pages (from-to)F280-F285
JournalAmerican Journal of Physiology - Renal Fluid and Electrolyte Physiology
Volume264
Issue number2 33-2
DOIs
StatePublished - 1993
Externally publishedYes

Keywords

  • proximal convoluted tubule

ASJC Scopus subject areas

  • Physiology

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    Yamaguchi, I., Jose, P. A., Mouradian, M. M., Canessa, L. M., Monsma, F. J., Sibley, D. R., Takeyasu, K., & Felder, R. A. (1993). Expression of dopamine D(1A) receptor gene in proximal tubule of rat kidneys. American Journal of Physiology - Renal Fluid and Electrolyte Physiology, 264(2 33-2), F280-F285. https://doi.org/10.1152/ajprenal.1993.264.2.f280