Expression of cloned β-endorphin gene sequences by Escherichia coli

John Shine, Ivy Fettes, Nancy C Y Lan, James Roberts, John D. Baxter

Research output: Contribution to journalArticle

42 Citations (Scopus)

Abstract

DNA coding for the opiate peptide β-endorphin has been cloned into bacterial plasmids in such a way as to direct the synthesis of a hybrid β-galactosidase/β-endorphin protein. This hybrid protein can readily be cleaved in vitro to release biologically active β-endorphin.

Original languageEnglish (US)
Pages (from-to)456-461
Number of pages6
JournalNature
Volume285
Issue number5765
DOIs
StatePublished - 1980
Externally publishedYes

Fingerprint

Endorphins
Escherichia coli
Galactosidases
Genes
Opioid Peptides
Proteins
Plasmids
DNA

ASJC Scopus subject areas

  • General

Cite this

Shine, J., Fettes, I., Lan, N. C. Y., Roberts, J., & Baxter, J. D. (1980). Expression of cloned β-endorphin gene sequences by Escherichia coli. Nature, 285(5765), 456-461. https://doi.org/10.1038/285456a0

Expression of cloned β-endorphin gene sequences by Escherichia coli. / Shine, John; Fettes, Ivy; Lan, Nancy C Y; Roberts, James; Baxter, John D.

In: Nature, Vol. 285, No. 5765, 1980, p. 456-461.

Research output: Contribution to journalArticle

Shine, J, Fettes, I, Lan, NCY, Roberts, J & Baxter, JD 1980, 'Expression of cloned β-endorphin gene sequences by Escherichia coli', Nature, vol. 285, no. 5765, pp. 456-461. https://doi.org/10.1038/285456a0
Shine J, Fettes I, Lan NCY, Roberts J, Baxter JD. Expression of cloned β-endorphin gene sequences by Escherichia coli. Nature. 1980;285(5765):456-461. https://doi.org/10.1038/285456a0
Shine, John ; Fettes, Ivy ; Lan, Nancy C Y ; Roberts, James ; Baxter, John D. / Expression of cloned β-endorphin gene sequences by Escherichia coli. In: Nature. 1980 ; Vol. 285, No. 5765. pp. 456-461.
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