Expression of cloned β-endorphin gene sequences by Escherichia coli

John Shine; Ivy Fettes; Nancy C.Y. Lan; James L. Roberts; John D. Baxter

doi:10.1038/285456a0

Expression of cloned β-endorphin gene sequences by Escherichia coli

John Shine, Ivy Fettes, Nancy C.Y. Lan, James L. Roberts, John D. Baxter

Research output: Contribution to journal › Article › peer-review

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Abstract

DNA coding for the opiate peptide β-endorphin has been cloned into bacterial plasmids in such a way as to direct the synthesis of a hybrid β-galactosidase/β-endorphin protein. This hybrid protein can readily be cleaved in vitro to release biologically active β-endorphin.

Original language	English (US)
Pages (from-to)	456-461
Number of pages	6
Journal	Nature
Volume	285
Issue number	5765
DOIs	https://doi.org/10.1038/285456a0
State	Published - 1980
Externally published	Yes

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title = "Expression of cloned β-endorphin gene sequences by Escherichia coli",

abstract = "DNA coding for the opiate peptide β-endorphin has been cloned into bacterial plasmids in such a way as to direct the synthesis of a hybrid β-galactosidase/β-endorphin protein. This hybrid protein can readily be cleaved in vitro to release biologically active β-endorphin.",

author = "John Shine and Ivy Fettes and Lan, {Nancy C.Y.} and Roberts, {James L.} and Baxter, {John D.}",

year = "1980",

doi = "10.1038/285456a0",

language = "English (US)",

volume = "285",

pages = "456--461",

journal = "Nature",

issn = "0028-0836",

publisher = "Nature Publishing Group",

number = "5765",

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T1 - Expression of cloned β-endorphin gene sequences by Escherichia coli

AU - Shine, John

AU - Fettes, Ivy

AU - Lan, Nancy C.Y.

AU - Roberts, James L.

AU - Baxter, John D.

PY - 1980

Y1 - 1980

N2 - DNA coding for the opiate peptide β-endorphin has been cloned into bacterial plasmids in such a way as to direct the synthesis of a hybrid β-galactosidase/β-endorphin protein. This hybrid protein can readily be cleaved in vitro to release biologically active β-endorphin.

AB - DNA coding for the opiate peptide β-endorphin has been cloned into bacterial plasmids in such a way as to direct the synthesis of a hybrid β-galactosidase/β-endorphin protein. This hybrid protein can readily be cleaved in vitro to release biologically active β-endorphin.

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U2 - 10.1038/285456a0

DO - 10.1038/285456a0

M3 - Article

C2 - 6250034

AN - SCOPUS:0019309907

SN - 0028-0836

VL - 285

SP - 456

EP - 461

JO - Nature

JF - Nature

IS - 5765

ER -

Expression of cloned β-endorphin gene sequences by Escherichia coli

Abstract

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