Abstract
This study determined the prevalence and the prognostic value of the expression of microtubule components in tumors of 19 patients with non small cell lung cancer receiving taxane-based regimens. Patient samples were stained with antibodies directed against total β tubulin, classes I, II and III β tubulin isotypes, δ2 alpha tubulin, τ protein, and the P-gp protein involved in the classical multidrug resistance phenotype. All tumors were stained with pan-β tubulin antibody and class I tubulin isotype. A majority of the tumor samples expressed class II and class III, although the percentage of positive cells varied significantly between tumors. δ2 alpha tubulin, τ protein and Pgp protein were found in only one tumor sample each. Progression-free survival was shorter (41 days) in patients whose tumors expressed high levels of class III tubulin isotype in comparison to patients with low levels (288 days, p=0.02). There were 2 responses to chemotherapy among 9 patients (22%) with high levels of class III tubulin vs. 6 among 10 patients (60%) with low levels of expression (Fisher exact test: p= 0.11). These data suggest that high expression of class III tubulin by tumor cells is associated with poor prognosis in patients with NSCLC receiving a taxane-based regimen.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 58-64 |
| Number of pages | 7 |
| Journal | Electronic Journal of Oncology |
| Issue number | 1 |
| State | Published - 2002 |
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ASJC Scopus subject areas
- Cancer Research
Cite this
Expression of class III b tubulin in non-small cell lung cancer is correlated with resistance to taxane chemotherapy. / Dumontet, Charles; Isaac, Sylvie; Souquet, Pierre Jean; Bejui-Thivolet, Françoise; Pacheco, Yves; Peloux, Nadine; Frankfurter, Anthony; Luduena, Richard; Perol, Maurice.
In: Electronic Journal of Oncology, No. 1, 2002, p. 58-64.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Expression of class III b tubulin in non-small cell lung cancer is correlated with resistance to taxane chemotherapy
AU - Dumontet, Charles
AU - Isaac, Sylvie
AU - Souquet, Pierre Jean
AU - Bejui-Thivolet, Françoise
AU - Pacheco, Yves
AU - Peloux, Nadine
AU - Frankfurter, Anthony
AU - Luduena, Richard
AU - Perol, Maurice
PY - 2002
Y1 - 2002
N2 - This study determined the prevalence and the prognostic value of the expression of microtubule components in tumors of 19 patients with non small cell lung cancer receiving taxane-based regimens. Patient samples were stained with antibodies directed against total β tubulin, classes I, II and III β tubulin isotypes, δ2 alpha tubulin, τ protein, and the P-gp protein involved in the classical multidrug resistance phenotype. All tumors were stained with pan-β tubulin antibody and class I tubulin isotype. A majority of the tumor samples expressed class II and class III, although the percentage of positive cells varied significantly between tumors. δ2 alpha tubulin, τ protein and Pgp protein were found in only one tumor sample each. Progression-free survival was shorter (41 days) in patients whose tumors expressed high levels of class III tubulin isotype in comparison to patients with low levels (288 days, p=0.02). There were 2 responses to chemotherapy among 9 patients (22%) with high levels of class III tubulin vs. 6 among 10 patients (60%) with low levels of expression (Fisher exact test: p= 0.11). These data suggest that high expression of class III tubulin by tumor cells is associated with poor prognosis in patients with NSCLC receiving a taxane-based regimen.
AB - This study determined the prevalence and the prognostic value of the expression of microtubule components in tumors of 19 patients with non small cell lung cancer receiving taxane-based regimens. Patient samples were stained with antibodies directed against total β tubulin, classes I, II and III β tubulin isotypes, δ2 alpha tubulin, τ protein, and the P-gp protein involved in the classical multidrug resistance phenotype. All tumors were stained with pan-β tubulin antibody and class I tubulin isotype. A majority of the tumor samples expressed class II and class III, although the percentage of positive cells varied significantly between tumors. δ2 alpha tubulin, τ protein and Pgp protein were found in only one tumor sample each. Progression-free survival was shorter (41 days) in patients whose tumors expressed high levels of class III tubulin isotype in comparison to patients with low levels (288 days, p=0.02). There were 2 responses to chemotherapy among 9 patients (22%) with high levels of class III tubulin vs. 6 among 10 patients (60%) with low levels of expression (Fisher exact test: p= 0.11). These data suggest that high expression of class III tubulin by tumor cells is associated with poor prognosis in patients with NSCLC receiving a taxane-based regimen.
UR - http://www.scopus.com/inward/record.url?scp=0037871319&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0037871319&partnerID=8YFLogxK
M3 - Article
AN - SCOPUS:0037871319
SP - 58
EP - 64
JO - Electronic Journal of Oncology
JF - Electronic Journal of Oncology
SN - 1292-8933
IS - 1
ER -