Expression from cloned DNA of biologically active glycoprotein C of herpes simplex virus type 1 in mammalian cells

N. Ghosh-Choudhury, M. Butcher, H. P. Ghosh

Research output: Contribution to journalArticlepeer-review

13 Scopus citations

Abstract

A DNA fragment of the herpes simplex virus type 1 genome encoding glycoprotein C (gC-1) has been cloned into different eukaryotic expression vectors for transient and stable expression of the glycoprotein in a number of cell lines. All of these expression vectors use a non-HSV promoter, such as the adenovirus major late promoter or murine leukaemia virus long terminal repeat promoter to express gC-1 in COS and CHO cells or 3T3 cells. The gC-1 protein synthesized was fully glycosylated with both N- and O-linked oligosaccharides. Synthesis of the mature 120K gC-1 glycoprotein involved partially glycosylated 100K and 105K proteins and the non-glycosylated 70K protein as intermediate molecules. Immunofluorescence studies showed that the expressed gC-1 was localized intracellulary in the nuclear envelope as well as on the cell surface. The expressed gC-1 was biologically active and could act as a receptor for the complement component C3b in the absence of other HSV proteins.

Original languageEnglish (US)
Pages (from-to)689-699
Number of pages11
JournalJournal of General Virology
Volume71
Issue number3
DOIs
StatePublished - 1990

ASJC Scopus subject areas

  • Virology

Fingerprint Dive into the research topics of 'Expression from cloned DNA of biologically active glycoprotein C of herpes simplex virus type 1 in mammalian cells'. Together they form a unique fingerprint.

Cite this