Expression and processing of small integrin-binding ligand N-linked glycoproteins in mouse odontoblastic cells

Shuo Chen, Lei Chen, Allen Jahangiri, Bo Chen, Yimin Wu, Hui Hsiu Chuang, Chunlin Qin, Mary MacDougall

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

Objective: Small integrin-binding ligand N-linked glycoproteins (SIBLINGs) are expressed in dentin and believed to control dentinogenesis. Five members of SIBLING family include bone sialoprotein (BSP), osteopontin (OPN), matrix extracellular phosphoglycoprotein (MEPE), dentin matrix protein 1 (DMP1) and dentin sialophosphoprotein (DSPP). These genes are clustered on chromosome 4q in humans and share similar biological features. DSPP and DMP1 are processed into given structural/functional fragments in rat and porcine. It still remains unclear whether these evidences occur in mouse and other SIBLING members are also processed into given fragments from their parent precursors. The aim of this study was to identify expression and processing of the five proteins in two mouse odontoblastic cell lines. Design: Two mouse odontoblastic cells were used to study expression and processing of the five SIBLING proteins by immunohistochemistry and Western blot analyses. Results: Immunohistochemistry study showed that all of the five SIBLING members were expressed within the cytoplasm and cellular processes in the mouse odontoblastic cell lines. Expression levels of DMP1 and DSPP were higher in differentiated mouse odontoblasts than undifferentiated mouse odontoblasts. Immunolabelling signal of DSP and MEPE was also detected within the nucleus in the two cell lines. Western blot assay indicated that all five members were processed into at least two fragments in these cells. Conclusions: These results suggest that different processed products and expression levels of the SIBLING proteins may play distinct biological functions in tooth development and mineralisation.

Original languageEnglish (US)
Pages (from-to)879-889
Number of pages11
JournalArchives of Oral Biology
Volume53
Issue number9
DOIs
StatePublished - Sep 2008

Fingerprint

Integrins
Glycoproteins
Dentin
Ligands
Odontoblasts
Proteins
Cell Line
Extracellular Matrix
Dentinogenesis
Western Blotting
Immunohistochemistry
Integrin-Binding Sialoprotein
Osteopontin
Tooth
Cytoplasm
Swine
Chromosomes
Genes
dentin sialophosphoprotein

Keywords

  • Dentin
  • Dentinogenesis
  • Odontoblasts
  • SIBLING
  • Teeth

ASJC Scopus subject areas

  • Dentistry(all)

Cite this

Expression and processing of small integrin-binding ligand N-linked glycoproteins in mouse odontoblastic cells. / Chen, Shuo; Chen, Lei; Jahangiri, Allen; Chen, Bo; Wu, Yimin; Chuang, Hui Hsiu; Qin, Chunlin; MacDougall, Mary.

In: Archives of Oral Biology, Vol. 53, No. 9, 09.2008, p. 879-889.

Research output: Contribution to journalArticle

Chen, Shuo ; Chen, Lei ; Jahangiri, Allen ; Chen, Bo ; Wu, Yimin ; Chuang, Hui Hsiu ; Qin, Chunlin ; MacDougall, Mary. / Expression and processing of small integrin-binding ligand N-linked glycoproteins in mouse odontoblastic cells. In: Archives of Oral Biology. 2008 ; Vol. 53, No. 9. pp. 879-889.
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AU - MacDougall, Mary

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AB - Objective: Small integrin-binding ligand N-linked glycoproteins (SIBLINGs) are expressed in dentin and believed to control dentinogenesis. Five members of SIBLING family include bone sialoprotein (BSP), osteopontin (OPN), matrix extracellular phosphoglycoprotein (MEPE), dentin matrix protein 1 (DMP1) and dentin sialophosphoprotein (DSPP). These genes are clustered on chromosome 4q in humans and share similar biological features. DSPP and DMP1 are processed into given structural/functional fragments in rat and porcine. It still remains unclear whether these evidences occur in mouse and other SIBLING members are also processed into given fragments from their parent precursors. The aim of this study was to identify expression and processing of the five proteins in two mouse odontoblastic cell lines. Design: Two mouse odontoblastic cells were used to study expression and processing of the five SIBLING proteins by immunohistochemistry and Western blot analyses. Results: Immunohistochemistry study showed that all of the five SIBLING members were expressed within the cytoplasm and cellular processes in the mouse odontoblastic cell lines. Expression levels of DMP1 and DSPP were higher in differentiated mouse odontoblasts than undifferentiated mouse odontoblasts. Immunolabelling signal of DSP and MEPE was also detected within the nucleus in the two cell lines. Western blot assay indicated that all five members were processed into at least two fragments in these cells. Conclusions: These results suggest that different processed products and expression levels of the SIBLING proteins may play distinct biological functions in tooth development and mineralisation.

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