Expression and function of a mislocalized form of peroxisomal malate dehydrogenase (MDH3) in yeast

L. McAlister-Henn, J. S. Steffan, K. I. Minard, S. L. Anderson

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Abstract

The malate dehydrogenase isozyme MDH3 of Saccharomyces cerevisiae was found to be localized to peroxisomas by cellular fractionation and density gradient centrifugation. However, unlike other yeast peroxisoreal enzymes that function in the glyoxylate pathway, MDH3 was found to be refractory to catabolite inactivation, i.e. to rapid inactivation and degradation following glucose addition. To examine the structural requirements for organellar localization, the Ser-Lys-Leu carboxyl-terminal tripeptide, a common motif for localization of peroxisomal proteins, was removed by mutagenesis of the MDH3 gene. This resulted in cytosolic localization of MDH3 in yeast transformants. To examine structural requirements for catabolite inactivation, a 12-residue amino-terminal extension from the yeast cytosolic MDH2 isozyme was added to the amino termini of the peroxisomal and mislocalized 'cytosolic' forms of MDH3. This extension was previously shown to be essential for catabolite inactivation of MDH2 but failed to confer this property to MDH3. The mislocalized cytosolic forms of MDH3 were found to be catalytically active and competent for metabolic functions normally provided by MDH2.

Original languageEnglish (US)
Pages (from-to)21220-21225
Number of pages6
JournalJournal of Biological Chemistry
Volume270
Issue number36
DOIs
StatePublished - 1995

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Malate Dehydrogenase
Yeast
Yeasts
seryl-lysyl-leucine
Isoenzymes
Density Gradient Centrifugation
Mutagenesis
Saccharomyces cerevisiae
Centrifugation
Fractionation
Refractory materials
Glucose
Genes
Enzymes
Degradation
Proteins

ASJC Scopus subject areas

  • Biochemistry

Cite this

Expression and function of a mislocalized form of peroxisomal malate dehydrogenase (MDH3) in yeast. / McAlister-Henn, L.; Steffan, J. S.; Minard, K. I.; Anderson, S. L.

In: Journal of Biological Chemistry, Vol. 270, No. 36, 1995, p. 21220-21225.

Research output: Contribution to journalArticle

McAlister-Henn, L. ; Steffan, J. S. ; Minard, K. I. ; Anderson, S. L. / Expression and function of a mislocalized form of peroxisomal malate dehydrogenase (MDH3) in yeast. In: Journal of Biological Chemistry. 1995 ; Vol. 270, No. 36. pp. 21220-21225.
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