Explant Methods for Epidermal Cell Culture

Susan M. Fischer, Aurora Viaje, Gerald D. Mills, Thomas J. Slaga

Research output: Contribution to journalArticle

19 Scopus citations

Abstract

Human skin epithelial cells grown in vitro from explants display the same organizational behavior found in vivo. Culture conditions found to encourage epidermal outgrowth from explants include minimal rather than complex media and supplementation of such media with 10% FCS, EGF, and hydrocortisone. The explant method provides a means of obtaining a large number of keratinocytes from a small piece of tissue. These primary cultures are useful in studies on the proliferation-differentiation process in skin and on the response of skin to various pharmacological agents, and in vitro transformation studies in which explants are potentially the best model for human carcinogenesis.

Original languageEnglish (US)
Pages (from-to)207-227
Number of pages21
JournalMethods in Cell Biology
Volume21
Issue numberC
DOIs
StatePublished - Jan 1 1980
Externally publishedYes

ASJC Scopus subject areas

  • Cell Biology

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    Fischer, S. M., Viaje, A., Mills, G. D., & Slaga, T. J. (1980). Explant Methods for Epidermal Cell Culture. Methods in Cell Biology, 21(C), 207-227. https://doi.org/10.1016/S0091-679X(08)60768-2