Exosome cofactors connect transcription termination to RNA processing by guiding terminated transcripts to the appropriate exonuclease within the nuclear exosome

Kyumin Kim, Dong Hyuk Heo, Iktae Kim, Jeong Yong Suh, Minkyu Kim

Research output: Contribution to journalArticlepeer-review

16 Scopus citations

Abstract

The yeast Nrd1 interacts with the C-terminal domain (CTD) of RNA polymerase II (RNApII) through its CTD-interacting domain (CID) and also associates with the nuclear exosome, thereby acting as both a transcription termination and RNA processing factor. Previously, we found that the Nrd1 CID is required to recruit the nuclear exosome to the Nrd1 complex, but it was not clear which exosome subunits were contacted. Here, we show that two nuclear exosome cofactors, Mpp6 and Trf4, directly and competitively interact with the Nrd1 CID and differentially regulate the association of Nrd1 with two catalytic subunits of the exosome. Importantly, Mpp6 promotes the processing of Nrd1-terminated transcripts preferentially by Dis3, whereas Trf4 leads to Rrp6-dependent processing. This suggests that Mpp6 and Trf4 may play a role in choosing a particular RNA processing route for Nrd1-terminated transcripts within the exosome by guiding the transcripts to the appropriate exonuclease.

Original languageEnglish (US)
Pages (from-to)13229-13242
Number of pages14
JournalJournal of Biological Chemistry
Volume291
Issue number25
DOIs
StatePublished - Jun 17 2016
Externally publishedYes

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

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